IL-2、IL-6对地塞米松诱导小鼠胸腺细胞凋亡的调节作用  

The regulation of cytokines on apoptosis induced by dexamethasone in mouse thymocyte

作　　者：刘玉涛[1] 殷金珠[1] 葛薇 白小薇[1] 

机构地区：[1]北京医科大学免疫系

出　　处：《中国免疫学杂志》1999年第7期299-301,共3页Chinese Journal of Immunology

摘　　要：目的：以Ｄｅｘ诱导小鼠胸腺细胞凋亡为模型，研究细胞因子（ＩＬ２、ＩＬ６）对小鼠胸腺细胞凋亡的调节作用。方法：应用ＰＩ法检测亚二倍体细胞，二苯胺法测定胸腺细胞片段化ＤＮＡ含量（％），ＤＮＡ凝胶电泳，流式细胞计分析胸腺细胞表型、测定高钙细胞百分比。结果：发现地塞米松（Ｄｅｘ）与小鼠胸腺细胞共同培养引起细胞凋亡，胸腺细胞减少以ＣＤ４＋ＣＤ８＋细胞最明显。细胞凋亡具有时间效应并与Ｄｅｘ浓度有关。３～４ｗ小鼠胸腺细胞对Ｄｅｘ的敏感性高于６～８ｗ小鼠。高浓度（＞２００Ｕ／ｍｌ）ＩＬ２或１００Ｕ／ｍｌＩＬ２与ＩＬ６（１００Ｕ／ｍｌ）协同可减轻Ｄｅｘ诱导的胸腺细胞凋亡，其片段化ＤＮＡ和亚二倍体细胞明显减少，胞浆Ｃａ２＋浓度降低，胸腺细胞亚群分布得到纠正。单独应用ＩＬ６不能改变Ｄｅｘ诱导的胸腺细胞凋亡。结论：地塞米松可促进小鼠胸腺细胞凋亡，高浓度（＞２００Ｕ／ｍｌ）ＩＬ２或１００Ｕ／ｍｌＩＬ２与ＩＬ６（１００Ｕ／ｍｌ）协同可减轻Ｄｅｘ诱导的胸腺细胞凋亡。Objective: IL2 and IL6 regulated the apoptosis of murine thymocytes induced by Dex .Methods: PI staining,DNA fragmentation,DNA gel electrophoresis,FACScan,and so on. Results: Apoptosis induced by Dex in mouse thymocytes has been shown that the number of thymocytes was decreased,especially CD4+CD8+cells.The apoptosis of thymocytes depends on the incubation time and the dose of Dex.The thymocytes from 24 weeks old mice are more sensitive to Dex than those from 68 weeks old mice. It has been shown show that high dose IL2(>200 U/ml) or low dose IL2(100 U/ml) with IL6(100 U/ml) could reduce the DNA fragmentation and the percentage of apoptotic mucleic (hypodiploid cells) in the thymocytes treated with Dex.Cytosolic calcium ion concentration and distribution of subpopulation of mouse thymocytes are corrected by these cytokines.IL6 alone has no effect on this apoptosis. Conclusion: Dex could induce the apoptosis of murine thymocytes,which could be reduced by high dose IL2(>200 U/ml) or low dose IL2(100 U/

关 键 词：细胞因子 胸腺细胞凋亡 IC-2 IL-6 

分 类 号：R392.12[医药卫生—免疫学]