鹿角方吸收成分对乳鼠心肌细胞胞浆游离钙浓度的影响  

EFFECTS OF ABSORBABLE COMPONENT OF LUJIAOFANG(ACL) ON CYTOSOLIC FREE CALCIUM CONCENTRATION IN CULTURED IMMATURE MYOCARDIAL CELLS

作　　者：李建军[1] 胡婉英[2] 董耀荣[2] 叶伟成[2] 

机构地区：[1]中山医科大学孙逸仙纪念医院,广州510120 [2]上海中医药大学附属曙光医院,上海200021

出　　处：《中国中医药科技》1999年第4期215-217,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：国家中医药管理局部分资助课题

摘　　要：目的:观察鹿角方吸收成分对培养乳鼠心肌细胞胞浆游离钙的作用,探讨其强心机制。方法:采用血清药理学方法,使用钙荧光探针Fura-Z/AM观察鹿角方药物血清对乳鼠心肌细胞胞浆游离钙的影响。结果:鹿角方药物血清可升高培养乳鼠心肌细胞胞浆游离钙浓度,其作用依赖于细胞外钙存在。结论:鹿角方吸收成分可促进细胞外Ca^(2+)内流,升高心肌细胞胞浆游离钙浓度,这与其增强心肌收缩有关。Objective: To observe the effects of absorbale components of Lujiaofang (ALC)on cytosolic free calcium [Ca2+ ]i concentration in cultured immature rat myocardial cells, and investigate its cardial enhancement mechanism. Methods:ACL- containing rat serum was collected by application seropharmacological method. [Ca2+ ]i concentration was measured by using calcium fluorescence probe Fura - 2/AM. Results: ACL - containing serum elevated the concentration of cytosolic [ Ca2+ ] i in immature rat myocardial cells in the condition of extrocellular Ca 2+ . Conclusion:Stimulation of ACL on Ca2 + intromission and elevation of myosolic[Ca2 + ]i concentration of myocardial cells are relation to enhancement of cardial constriction.

关 键 词：鹿角方 药理学 心肌细胞 游离钙 中药药理 

分 类 号：R289.5[医药卫生—方剂学] R285.5[医药卫生—中药学]