复方银杏叶胶囊对肝损伤大鼠CYP2E1、CYP3A4的影响  

Influence of the compound Ginkgo Biloba on CYP2E1 and CYP3A4 enzyme activity in hepatic injuried rats

作　　者：刘亚锋[1] 潘苏华[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《东南国防医药》2011年第2期100-103,共4页Military Medical Journal of Southeast China

基　　金：江苏省中医局基金项目(H-024)

摘　　要：目的研究复方银杏叶胶囊(CGB)对酒精性肝损伤大鼠CYP2E1、CYP3A4活性的影响。方法正常组和酒精性肝损伤模型组均以CGB[(250 mg/(kg.d)]灌胃,分别在灌胃CGB前及灌胃1周后,灌胃探针药氯唑沙宗(50 mg/kg)及氨苯砜(20 mg/kg),于探针药灌后24 h内不同时间点采血,测定各探针药血药浓度。结果灌胃CGB前,模型组氯唑沙宗和氨苯砜的AUC0-24、Cmax均显著低于正常组(P<0.05或P<0.01)。灌胃CGB后,模型组氯唑沙宗和氨苯砜的AUC0-24、Cmax均较灌胃前显著升高(P<0.05或P<0.01);且氯唑沙宗的t1/2灌胃CGB后明显高于灌胃前(P<0.05)。结论 CGB能够明显抑制酒精性肝损伤大鼠CYP2E1、CYP3A4酶活性。Objective To observe the effect of the compound Ginkgo Biloba（CGB） on CYP2E1 and CYP3A4 activity in hepatic alcohol-injuried rats.Methods CGB was orally administrated to the normal and the alcohol-injured hepatic injury model rats.Two group rats underwent 2-cycle pharmacokinetic experiments before and after being treated with CGB（250 mg·kg^-1·d^-1） for 7 days,in which the rats were concomitantly administered Chlorzoxazone（50 mg/kg） and Dapsone（20 mg/kg） by gastrogavage followed by blood-withdrawing from orbital bleeding at different intervals within 24 hours.Results Before being treated with CGB,compared with normal control group,the AUC0-24 and Cmax of Chlorzoxazone and Dapsone in the model group were decreased significantly（P〈0.05,P〈0.01）.However,the AUC0-24,and Cmax of Chlorzoxazone and Dapsone after being treated with CGB in the model group were both larger than those before treatment（P〈0.05,P〈0.01）,and the t1/2 of Chlorzoxazon than that that before treatment.Conclusion CGB could inhibit activities of CYP2E1 and CYP3A4 in alcohol-injuried rats.

关 键 词：酒精性肝损伤 银杏叶提取物 CYP2E1 CYP3A4 

分 类 号：R969.1[医药卫生—药理学]