棉花黄萎病拮抗细菌HMB-1005鉴定及定殖  

Studies on identification and colonization of antagonistic bacteria HMB-1005 to cotton Verticillium dahliae

作　　者：郭慧娟[1] 李术娜[1] 李红亚[1] 王全[1] 王树香[1] 王伟[1] 朱宝成[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《中国土壤与肥料》2011年第2期84-87,共4页Soil and Fertilizer Sciences in China

基　　金：河北省自然科学基金项目(398152)

摘　　要：以大丽轮枝菌(Verticillium dahliae)为指示菌,对从棉田土壤中已经分离筛选到的细菌HMB-1005进行形态、19项生理生化特征及16S rDNA试验,结果表明,HMB-1005为解淀粉芽孢杆菌(Bacillus amyloliquefaciens);通过灭菌土和非灭菌土进行盆栽试验,拮抗菌HMB-1005芽孢液浸种、土壤全部混菌和部分混菌3种处理,经过5个月的盆栽试验表明,拮抗细菌HMB-1005能够在灭菌土和非灭菌土中定殖,且定殖数量达106cfu/g土左右,并仍能保持较高的抑菌活性。通过对棉苗根内细菌的分离,证实拮抗细菌HMB-1005能在棉花根内定殖,数量达到103cfu/g。实验结果显示,灭菌土的拮抗菌数高于非灭菌土,并且3种处理方式差异不明显,在实际生产应用中,建议使用浸种方法。In this study,determination of antagonistic activity was done to the strain HMB-1005 from the cotton fields with the Verticillium dahliae strains as the instructions,characteristics of strain HMB-1005 equates well with Bacillus amyloliquefaciens at the level of species in morphological,19 physiological biochemical items and 16S rDNA sequence.This test required sterile soil and non-sterile soil in two soil samples,this test need two soil samples of three different treatment that was：The antagonistic bacterium bacillus soaking liquid,all mixed soil and part of soil for plants mixed.The purpose of this paper was to study antagonistic bacteria HMB-1005 in the soil and in the colonization of cotton roots and rhizosphere colonization of the situation.The results showed that antagonistic bacteria HMB-1005 in non-sterilized soil and sterilized soil colonization could reproduce,and the quantity achieved 106cfu/g.Through revovery bacteria of cotton roots,confirmed the antagonistic bacteria in the HMB-1005 designated cotton root colonization,number of 103cfu/g.This experiment indicated the antibacterial number in antiseptic was higher than the non-antiseptic earth,and the difference during three processing modes was not obviously.Considered the various factor,minded that the first method would be better.

关 键 词：棉花黄萎病 拮抗细菌 定殖能力 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]