揉髌手法对兔膝关节软骨细胞凋亡及增殖细胞核抗原表达的影响  

Effects of roubin-manipulation on knee joint chondrocyte apoptosis and proliferating cell nuclear antigen expression in rabbits

作　　者：欧国建[1] 戴七一[2] 覃学流[2] 林光琪[3] 刘靖[3] 滕居赞[2] 姚平[2] 朱江龙[2] 容向宾[2] 韩杰[2] 辛晓东[3] 袁经阳[3] 

机构地区：[1]广西中医学院附属贺州医院骨伤科,广西壮族自治区贺州市542500 [2]广西中医学院附属瑞康医院骨四肢关节科,广西壮族自治区南宁市530011 [3]广西中医学院,广西壮族自治区南宁市530001

出　　处：《中国组织工程研究与临床康复》2010年第50期9387-9390,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金项目(30860359);广西区科技厅自然科学基金项目(0832178)资助~~

摘　　要：背景:有研究表明手法的力学刺激作用和关节被动活动可促进促进关节周围组织的自我修复,但手法治疗对膝关节软骨细胞凋亡和增殖的影响目前未见报道。目的:用揉髌手法施术于骨内高压型动物的右下肢,探讨对兔膝关节软骨细胞凋亡和增殖细胞核抗原表达的影响。方法:将新西兰兔随机分为5组:揉髌手法组、玻璃酸钠组和模型组采用手术结扎股静脉,臀下静脉、大隐静脉方法建立右下肢骨内高压型模型;假手术组予以手术暴露但不结扎相应血管;正常对照组不作任何处理。造模1周后,揉髌手法组采用揉髌手法连续治疗5周共17次;玻璃酸钠组每周右膝关节内注射1次,连续5周。于造模后12周末分别取所有动物右膝胫骨平台软骨组织,采用原位末端标记法观察软骨细胞凋亡,免疫组化法观察增殖细胞核抗原的表达。结果与结论:揉髌手法组软骨细胞凋亡率低于模型组(P<0.01)。揉髌手法组增殖细胞核抗原表达率高于模型组(P<0.01)。结果表明揉髌手法治疗可以降低实验兔膝关节软骨细胞凋亡率及提高增殖细胞核抗原表达率。BACKGROUND： Studies have demonstrated that manipulation and joint passive movement can promote self-repair of surrounding tissues. However, it is poorly understood whether manipulation has effect on knee joint chondrocyte apoptosis and proliferating cell nuclear antigen expression （PCNA）. OBJECTIVE： To observe the effect of roubin-manipulation on knee joint chondrocyte apoptosis and PCNA expression.METHODS： New Zealand rabbits were randomly divided into 5 groups： manipulation group, sodium hyaluronate group and model contrast group were established high intraosseous pressure models of right leg by ligating the vena femoralis, gluteae inferiors or great saphenous vein; sham surgery group only cut out the skin and the normal group. One week later, the manipulation group was treated with roubin-manipulation for 5 weeks with 17 times. The sodium hyaluronate was injected into the intra-articular of sodium hyaluronate group once a week for 5 weeks. Cartilage sections of right knee-joint were analyzed by in situ end labeling and immunohistochemistry at the end of 12 weeks. RESULTS AND CONCLUSION： Compared with the model group, the apoptosis cell index of the manipulation group was smaller （P0.01） but the PCNA expression was greater （P0.01）. The results demonstrated that roubin-manipulation can decrease the apoptosis of chondrocyte and increase chondrocyte proliferation.

关 键 词：骨性关节炎 揉髌手法 关节软骨 细胞凋亡 增殖细胞核抗原 组织构建 

分 类 号：R318[医药卫生—生物医学工程]