丝素蛋白/羟基磷灰石材料复合人胎盘间充质干细胞修复桡骨节段性骨缺损  

Human placenta mesenchymal stem cells seeded on a silk fibroin/hydroxyapatite scaffold for repair of radius segmental bone defects

作　　者：钱寒光[1] 苗宗宁[1] 赵基栋[1] 彭玮[1] 

机构地区：[1]无锡市第三人民医院修复重建科,江苏省无锡市214041

出　　处：《中国组织工程研究与临床康复》2010年第51期9507-9511,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:丝素蛋白用于骨重建和再生,表现出机械稳定性和持久性。但单纯的丝素蛋白存在一定的缺陷,在含水量极低时易于破碎,在低湿环境应用时强度不够,通过与其他多聚物的复合可以进一步改进丝素蛋白的结构和特性。目的:观察胎盘间充质干细胞联合丝素蛋白/羟基磷灰石材料修复节段性骨缺损的效果。方法:制作兔桡骨中段1.5cm长骨缺损模型,以数字表法随机分为实验组(植入BrdU标记人胎盘间充质干细胞/丝素蛋白/羟基磷灰石)和对照组(植入丝素蛋白/羟基磷灰石)。植入后4,8,12周行大体、组织学与X射线观察,评分比较骨缺损修复情况。结果与结论:胎盘间充质干细胞与丝素蛋白/羟基磷灰石材料复合培养植入后,4周时新骨已开始形成,8周时骨缺损部分修复,12周时有部分新生骨组织形成板层骨,骨小梁形成,内可见大量成骨细胞,而对照组支架材料降解较慢。植入后第12周实验组影像学检测骨缺损区完全修复,皮质连续,塑型完全,不易分辨。对照组缺损区基本修复,但塑型不佳,缺损区易分辨,实验组新骨生成优于对照组。说明丝素蛋白/羟基磷灰石材料与胎盘间充质干细胞联合移植能够较好修复兔桡骨缺损。BACKGROUND：Silk fibroin can be used for bone reconstruction and regeneration,showing the mechanical stability and durability.Silk fibroin alone has some shortcomings,it s easily broken at very low water content,and not enough strong in low humidity environments,compounding with other polymer can further improve the silk fibroin structure and properties.OBJECTIVE：To observe the effect of placenta mesenchymal stem cells （PMSCs） combined with silk fibroin/hydroxyapatite （SF/HA） materials in repairing rabbit bone defects.METHODS：A 15-mm length of radius defect model was established in rabbits,which were randomly divided into experimental group and control group,implanted with BrdU-labeled PMSCs/SF/HA and SF/HA respectively.The gross observation,histological and radiographic examinations were analyzed on 4,8,and 12 weeks after transplantation to evaluate bone defects repair.RESULTS AND CONCLUSION：After PMSCs/SF/HA implantation,new bone formation at 4 weeks,bone defects were partially repaired at 8 weeks,and some new bone tissues formed lamellar bone and bone trabecular at 12 weeks,with a large amount of osteobloasts were seen,while the scaffolds degraded slowly in the control group.X-ray observation of the experiment group at 12 weeks after operation showed the bone defects were completely repaired,with continuous cortex,complete molding and cannot be distinguished.In control group,the defects were almost repaired,but the molding was poor and the defects can be detected.The new bone formation in the experimental group was better than that in control group.PMSCs/SF/HA composites transplantation can repair the radius bone defect in rabbits.

关 键 词：胎盘间充质干细胞 丝素蛋白 羟基磷灰石 组织工程 骨 

分 类 号：R318[医药卫生—生物医学工程]