同种异体脱钙骨基质的组织工程学特性  

Tissue engineered characterization of allogenic demineralized bone matrix

作　　者：冯万文[1,2] 夏亚一[3] 党跃修[3] 杨广智[1,2] 李广梅[1,2] 李小民[1,2] 柴玲霞[1,2] 常伶文[1,2] 翟德忠[1,2] 崔建国[1,2] 王晓红[1,2] 武茹[1,2] 李婷[1,2] 郭侃锁[1,2] 

机构地区：[1]徐州医学院附属连云港市东方医院骨科 [2]连云港市涉外医院骨科,江苏省连云港市222042 [3]兰州大学第二医院骨科,甘肃省兰州市730030

出　　处：《中国组织工程研究与临床康复》2010年第51期9512-9516,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：江苏省连云港市卫生局资助项目(09014)~~

摘　　要：背景:由骨衍生的支架材料无论形态学和力学特征,具有合成材料无可比拟的优势,脱钙骨基质具有和自体骨最接近的三维结构,同时以Ⅰ型胶原为主,胶原是细胞黏附和生长的良好支架。目的:从组织工程学角度研究同种异体脱钙骨基质的生物学特性,探讨其作为软骨组织工程支架材料的可行性。方法:据Urist描述的方法制备青紫蓝兔同种异体脱钙骨基质,扫描电镜观察脱钙骨基质的超微结构,测定其孔径、孔隙率和降解率,测定同种异体脱钙骨基质与骨髓间充质干细胞的黏附率,兔体内植入法评价同种异体脱钙骨基质的组织相容性。结果与结论:脱钙骨基质呈多孔海绵状三维结构,孔径在210～320μm之间,孔隙率为92%,体外降解12周降解率达90%以上,脱钙骨基质与骨髓间充质干细胞共培养第2,4,6天的黏附率分别为(51.50±2.30)%,(94.13±2.14)%和(87.24±1.75)%。兔体内植入6周后脱钙骨基质周围界面未引起明显的炎症和排斥反应,并形成软骨样结构和少量骨组织。说明脱钙骨基质具有适宜的三维多孔结构,降解时间和软骨形成时间同步,同种异体脱钙骨基质与种子细胞黏附率高,与细胞组织相容性好,能满足软骨组织工程对支架材料的要求,是理想的软骨组织工程的支架材料。BACKGROUND：Scaffold materials derived from bone have incomparable advantages compared with synthesized materials,regarding morphological or mechanical characterization.Demineralized bone matrix represents three-dimensional structure proximal to autogenous bone,it mainly contains Ⅰ-type collagen that is a good scaffold for cells adhesion and growth.OBJECTIVE：To study the biological characteristics of allogenic demineralized bone matrix （DBM） as viewed from tissue engineering,and to investigate the feasibility of DBM as a scaffold of cartilage tissue engineering.METHODS：DBM was prepared from chinchilla rabbits according to the methods described by Urist.Ultrastructures of DBM were observed by scanning electron microscope,the pore diameter,porosity and degradable rate of DBM were determined.Adhesive rate of bone marrow-derived mesenchymal stem cells into allogenic DBM was detected.Histocompatibility of allogenic DBM was evaluated by means of DBM implanting in rabbits.RESULTS AND CONCLUSION：DBM represented three-dimensional spongy-like structure with pores.Pore diameter of DBM ranged from 210μm to 320μm,mean porosity was 92%.Degradable rate of DBM in vitro at 12 weeks achieved over 90%.Adhesive rate of bone marrow-derived mesenchymal stem cells into allogenic DBM were （51.50±2.30）%,（94.13±2.14）% and （87.24±1.75）% at 2,4 and 6 days respectively after coculture.After allogenic DBM were implanted in rabbits at 6 weeks,interface between DBM and muscle tissue had no evident signs of inflammatory and rejective reaction,cartilage-like and bone tissue were also formed.DBM represents a three-dimensional porous structure which fits for adhesion and proliferation of seeding cells.Biodegradation of DBM synchronizes cartilage formation in phase.Allogenic DBM shows excellent adhesive activity and biocompatibility with cells,it can satisfy the requests of cartilage tissue engineering for scaffold materials,thus considered as an ideal scaffold material for cartilage tissue engineering.

关 键 词：脱钙骨基质 软骨 组织工程 支架材料 生物学特性 生物材料 

分 类 号：R318[医药卫生—生物医学工程]