羧甲基壳聚糖温敏凝胶的制备及其毒性实验  

Preparation and cytotoxicity of carboxymethyl chitosan thermosensitive hydrogel

作　　者：彭伟[1] 穆玉[1] 

机构地区：[1]华北煤炭医学院口腔系,河北省唐山市063000

出　　处：《中国组织工程研究与临床康复》2010年第51期9591-9594,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：河北省科技公关项目(07275524);甲壳素及其衍生物诱导人牙周膜细胞骨化分化的影响;河北省中医药管理局2009年度中西医结合科研计划项目(2009056);大黄壳聚糖温敏凝胶治疗牙周病的实验研究~~

摘　　要：背景:与壳聚糖相比,羧甲基壳聚糖的水溶性提高,且安全、无毒、无害,具有成膜性、保湿性、生物相容性好的特点,是一种良好的药物载体。目的:自制羧甲基壳聚糖温敏凝胶,了解其对小鼠肺成纤维细胞L929的毒性作用。方法:利用羧甲基壳聚糖与甘油磷酸盐互配,制备羧甲基壳聚糖温敏凝胶。采用四甲基偶氮唑盐法测定50,10,2,0.4,0.08倍的羧甲基壳聚糖温敏凝胶浸提液对体外培养L929细胞的细胞毒性,并设定阳性与阴性对照组。测定加样后24,48,72,96h的A值,计算细胞相对增殖率,评定细胞毒性等级。结果与结论:各组细胞不同时间点相对增殖率均在103%～228%之间,各浓度羧甲基壳聚糖温敏凝胶材料浸提液的细胞毒性均为0级。提示羧甲基壳聚糖温敏凝胶对L929细胞无毒性作用,且有良好的生物相容性,在牙周病治疗中具有潜在的应用价值。BACKGROUND：Compared with chitosan,carboxymethyl chitosan is safe,nontoxic,antigenic-free,and has higher water-solubility,membrane-forming,moisture-retention capacity and good biocompatibility,it is a good drug carrier.OBJECTIVE：To produce carboxymethyl chitosan thermosensitive hydrogel,and to evaluate its cytotoxicity on mouse lung fibroblasts （L929）.METHODS：The carboxymethyl chitosan mixed with glycerophosphate salt to produce the carboxymethyl chitosan thermosensitive hydrogel.MTT colorimetric assay was used to determine the cytotoxicity of L929 cells at different concentrations （50,10,2,0.4,0.08） of carboxymethyl chitosan thermosensitive hydrogel,positive and negative control groups were also set.Absorbance values were detected at 24,48,72,96 hours after adding the sample,the relative growth rates were counted and cytotoxicity grade was assessed.RESULTS AND CONCLUSION：The relative growth rates were ranged from 103% to 228% at different time points and cytotoxicity grade of different concentrations of the carboxymethyl chitosan thermosensitive hydrogel was 0.The carboxymethyl chitosan thermosensitive hydrogel shows no cytotoxicity on L929 and has good biocompatibility,it is potential in treatment of periodontal diseases.

关 键 词：羧甲基壳聚糖 L929细胞 牙周病 温敏凝胶 细胞毒性 生物材料 

分 类 号：R318[医药卫生—生物医学工程]