胶原-肝素自组装多层膜修饰钛表面  

Surface modification of titanium by collagen and heparin self-assembly multilayers

作　　者：李全辉[1] 徐祝军[2] 李全利[2] 沈军[2] 张维波[2] 

机构地区：[1]皖南医学院附属弋矶山医院骨一科,安徽省芜湖市241001 [2]安徽医科大学口腔医学院,安徽省合肥市230032

出　　处：《中国组织工程研究与临床康复》2010年第51期9595-9600,共6页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：安徽省高等学校自然科学基金重点项目(KJ2009A169):静电自组装牙种植体表面生物化修饰的研究;国家自然科学基金(307724400):自组装仿生构建可降解缓释活性复合骨组织修复材料的研究;安徽省科技攻关项目项目(08010302196):生物活性缓释制剂诱导牙周组织再生材料和构建骨缺损材料的临床前研究~~

摘　　要：背景:对钛表面进行生物化修饰,改善钛表面的生物相容性是目前钛表面改性研究的热点之一。目的:采用层层自组装技术,在钛表面构建胶原-肝素多层膜,实现钛表面的细胞外基质化修饰,以改善钛的细胞相容性。方法:首先采用NaOH处理钛基材,获得多孔、负电荷的钛表面;然后吸附1层正电荷的聚赖氨酸;最后,多次交替吸附负电荷的肝素和正电荷的胶原,形成以胶原为最外层的多层膜结构。通过漫反射红外光谱、扫描电镜、原子力显微镜、接触角测量对多层膜进行表征。并与骨髓基质细胞共培养,考察细胞的黏附、增殖情况。结果与结论:漫反射红外光谱、原子力显微镜、扫描电镜、接触角测量结果表明肝素-胶原多层膜逐渐形成,此涂层可促进成骨髓基质细胞的黏附、增殖和分化。肝素-胶原多层膜有望成为一种新型的生物化钛表面,从而改善钛表面的生物相容性。BACKGROUND：The biological surface modification for the titanium and improving the biocompatibility of titanium surface is one of the hot spots in research of titanium surface modification.OBJECTIVE：Collagen and heparin multilayers were coated on pure titanium using a layer-by-layer self-assembly technique,to achieve extracellular matrix modification of the titanium surface and to improve the cytocompatibility of titanium.METHODS：The film growth was initialized by depositing one layer of positively charged poly-L-lysine on the NaOH-treated titanium substrate （porous and negatively charged surface）.Then,the film was formed by the alternate deposition of negatively charged heparin and positively charged chitosan via electrostatic interactions of polyelectrolytes,and terminated with an outermost layer of collagen.The chemical composition,surface topography as well as roughness of the multilayers were investigated by using diffuse reflection-fourier transform infrared spectroscopy,scanning electron microscope,atomic force microscope,and contact angle measurement respectively.We cultured bone marrow stromal cells on the surface of modified-titanium and pure titanium respectively to observe its adhesion and proliferation.RESULTS AND CONCLUSION：The analysis of diffuse reflection-fourier transform infrared spectroscopy,scanning electron microscope,atomic force microscope,and contact angle measurement confirmed that,heparin-collagen multilayers formed gradually on the titanium surface and could improve cell adhesion,proliferation and differentiation.The heparin-collagen multilayers is a potential biological titanium surface to improve the biocompatibility of titanium surface.

关 键 词：钛 表面改性 层层自组装 胶原 肝素 

分 类 号：R318[医药卫生—生物医学工程]