一种新的代谢综合征大鼠模型的建立  

Established a new rat model of metabolic syndrome

作　　者：龙惠东[1,2] 林云恩[3] 曾昭华[2] 梁丽英[2] 蓝忠[2] 张海军[2] 

机构地区：[1]广州医学院附属肿瘤医院ICU [2]广州医学院第一附属医院内科心脏病研究室 [3]广州医学院第一附属医院病理科

出　　处：《中国临床药理学与治疗学》2010年第12期1326-1329,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：广东省科技计划项目(2009B030801359);广东省自然基金(9151018201000020)

摘　　要：目的:构建、评价高盐高脂饲养诱导的代谢综合征大鼠(MS)动物模型。方法:雄性Wistar大鼠,于哺乳期(3周龄)后,随机分为3组(每组12只):正常盐组(0.5%NaCl,NS)、高盐组(4%NaCl,HS)和高盐高脂组(49%fat+4%NaCl,FS),持续饲养18周,测体重,颈总动脉插管直接测血压,血浆测血脂指标TC、TG、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C),测血清胰岛素和血糖值。结果:HS、FS组较NS组大鼠血压明显增高(P<0.01),而HS组与FS组之间差异无统计学意义;FS组较NS组大鼠血脂明显增高,以TC、TG、LDL-C为主,三者均P<0.01,HDL-C无明显改变(P>0.05),HS组与NS组之间差异无统计学意义;FS组大鼠胰岛素抵抗指数较NS组明显升高(P<0.01),HS组与NS组之间差异无统计学意义;FS组较NS组大鼠体重明显增加(P<0.01),HS组与NS组之间差异无统计学意义。结论:长期高盐高脂饲养可诱导出符合或接近临床的代谢综合征大鼠模型。AIM：To establish and evaluate a rat model of metabolic syndrome induced by high fat and salt diet. METHODS：Wistar rats,aged 3 weeks,which were divided into three groups in randomized manner：control with normal salt diet（0.5% NaCl,NS）,high salt diet（4% NaCl,HS）,fat and salt-enriched diet（49%fat＋4%NaCl,FS）,12 rats in each group for 18 weeks.Then the bodyweights were measured.The right carotid artery was cannulated for direct blood pressure measurement.Insulin was measured in plasma sample by enzyme linked immuno sorbent assay（ELISA）.Other remaining plasma samples were obtained for total cholesterol concentra tion（TC）,triglyceride（TG）,low density lipoprotein cholesterol（LDL-C）,high density lipoprotein cholesterol（HDL-C）,blood glucose（GLU）.RESULTS：Compared with the NS and HS group,the body weight,blood pressure,insulin resistence index and triglycerol levels were all significantly increased in the FS group.But there was no significant difference between the NS and HS group.CONCLUSION：The fat and salt-enriched diet given to the rats can induce a metabolic syndrome,which corresponds or is analogous to the clinical metabolic syndrome.

关 键 词：代谢综合征 盐 脂肪 动物模型 大鼠 

分 类 号：R956.2[医药卫生—药学]