主要组织相容性复合物Ⅰ类链相关基因在肠组织中的表达及与溃疡性结肠炎的关系  

The study of major histocompatibility complex class Ⅰ related genes expression in colonic mucosa tissue and the correlation with ulcerative colitis

作　　者：葛柳青[1] 姜挺[1] 赵杰[1] 陈志涛[1] 周峰[1] 夏冰[1] 

机构地区：[1]武汉大学中南医院消化科,430071

出　　处：《中华消化杂志》2010年第11期811-813,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目（30570834）

摘　　要：目的 探讨主要组织相容性复合物Ⅰ类链相关基因（MIC）在溃疡性结肠炎（UC）发病中所起的作用.方法 采用荧光定量实时-聚合酶链反应（RT-PCR）方法检测34例UC患者和17例正常人肠黏膜组织中MICA和MICB及其受体NKG2D mRNA表达水平的差异.采用激光共聚焦显微镜观察肠黏膜组织中MICA分子的表达定位.结果 UC组MICA、MICB及NKG2DmRNA水平的表达量（分别为3.5408±2.6658、8.9879±3.2893和2.4395±0.8147）均显著高于正常对照组（分别为1.0477±0.7201、4.6293±1.2616和1.1624±0.3954,P值分别=0.0053、0.0039和0.0078）.免疫荧光共聚焦显微镜观察显示,MICA分子在肠上皮细胞胞膜中表达.结论 UC患者肠黏膜组织中MICA、MICB及其受体NKG2D的mRNA表达水平均增高,提示MIC基.因在UC局部起重要作用.Objective To investigate the function of major histocompatibility complex （MHC）class Ⅰ-related gene （MIC） in ulcerative colitis （UC） pathogenesis. Methods The difference of MICA, M ICB and their ligand NKG2D genes expression in colonic mucosa tissue of 34 UC patients and 12 healthy people were determined by fluorescent real-time reverse-transcriptase polymerase chain reaction （RT-PCR） and the expression location of M ICA in colonic mucosa tissue was obtained by laser scanning confocal microscope. Results The mRNA level of MICA, MICB and NKG2D expression in UC groups （3. 5408±2. 6658, 8. 9879±3. 2893 and 2. 4395±0. 8147 accordingly） was significantly higher than that in the healthy controls （ 1. 0477 ± 0. 7201, 4. 6293 ± 1. 2616 and 1. 1624±0. 3954 accordingly） （P = 0.0053, 0.0039 and 0. 0078 accordingly）. It suggested that MICA was expressed in colonic epithelia cell membranes by laser scanning confocal microscopy.Conclusion The mRNA level of MICA, M ICB, and their ligand, NKG2D expression were all up regulated in the colonic mucosa of UC patients, which indicated MIC gene might perform important local function in UC.

关 键 词：结肠炎 溃疡性 基因 主要组织相容性复合物Ⅰ类 NKG2D 

分 类 号：R686[医药卫生—骨科学]