血清雌激素水平与结肠黏膜组织错配修复基因表达水平的关系  

The correlation between serum estrogen level and the expression of mismatch repair genes in colonic mucosa

作　　者：金鹏[1] 盛剑秋[1] 陆晓娟[1] 付蕾[1] 孟晓明[1] 王昕[1] 韩英[1] 李世荣[1] 

机构地区：[1]北京军区总医院消化科,100700

出　　处：《中华消化杂志》2010年第11期814-818,共5页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目（30940086）

摘　　要：目的 研究体内雌激素对结肠黏膜组织错配修复基因表达的影响.方法 共选取结肠镜检查正常的健康人42名.于结肠镜检查前0.5 h抽取静脉血测定血清雌二醇（E2）水平,结肠镜下于右半结肠取肉眼观正常的结肠黏膜组织,以半定量逆转录（RT）-聚合酶链反应（PCR）和免疫组化染色法评定DNA错配修复基因hMLH1、hMSH2的表达量,分析血清E2水平与结肠黏膜hMLH1和hMSH2表达量的关系.结果 正常人血清E2水平与结肠黏膜组织hMLH1基因的表达量之间存在双峰曲线关系,当血清E2≥45 pg/ml时,hMLH1的表达量与血清E2水平正相关（mRNA相对表达量：P=0.003、r=0.701;免疫组化阳性指数：P=0.000,r=0.874）.但血清E2水平与hMSH2表达量间无关联.结论 体内高雌激素水平能增强结肠黏膜组织错配修复基因hMLH1的表达.Objective To investigate the effects of estrogen on mismatch repiar gene expression in colonic mucosa in vivo. Methods A total of 42 healthy individuals underwent colonoscopy were enrolled in the study. Half an hour before colonoscopy examination, blood sample was taken for determining the serum estradiol （E2） level. N ormal colonic mucosal tissues determined by naked eye under colonoscopy examination were taken in the right hemi colon to detect HMLH1 and hMSH2 gene expression by semi-quantitative RT-PCR and immunohistochemistry staining. Then the correlation of serum E2 levels with hMLH1 and hMSH2 expression in colonic mucosa was analyzed. Results A bimodal curve was presented for the correlation between serum E2 level in healthy individuals and hMLH1 expression in colonic mucosa. A strong positive correlation of E2 level with hMLH1 expression in normal colonic mucosa was observed when serum E2 level was more than 45 pg/ml （For mRNA, P=0. 003, r=0. 701; for immunohistochemistry positivity index, P=0. 000, r=0. 874）.However there was no correlation between E2 level and hMSH2 expression. Conclusion High serum E2 level might increase the hMLH1 gene expression in colonic mucosa in vivo.

关 键 词：雌激素 错配修复 结直肠癌 

分 类 号：R686[医药卫生—骨科学]