2009年广东省新型甲型H1N1流行性感冒病毒神经氨酸酶基因进化分析  

Evolutionary analysis of neuraminidase gene of novel influenza virus A/H1N1 in 2009 pandemic in Guangdong Province

作　　者：肖红[1] 管大伟[1] 邹丽容[1] 张欣[1] 倪汉忠[1] 柯昌文[1] 

机构地区：[1]广东省疾病预防控制中心微生物检验所,广州510300

出　　处：《中华传染病杂志》2010年第12期727-732,共6页Chinese Journal of Infectious Diseases

基　　金：基金项目：“十一五”医学重点专科资助项目[粤卫（2008）50号];广东省科技计划资助项目[粤科函（2008）1216号]

摘　　要：目的探讨2009年广东省新型甲型H1N1流行性感冒（流感）病毒神经氨酸酶（NA）基因的进化及NA基因编码蛋白抗原性、酶活性位点、糖基化位点变异情况。方法从2009年广东省新型甲型H1N1流感患者中分离到病毒毒株共69株，提取病毒总RNA，RT—PCR扩增NA基因，并测序分析；同时从美国国立生物技术信息中心基因库检索获得52株不同年代、不同地域甲型流感病毒NA基因序列，用MEGA4．0软件进行基因进化分析和氨基酸序列分析。结果2009年广东省新型甲型H1N1流感病毒NA基因与禽H5N1流感病毒同源性较高，为87．1％，潜在抗原位点氨基酸分布相同；所有毒株的酶活性中心位点高度保守；具有8个糖基化位点，其中5个位点有不同程度的氨基酸替换，但与2001年禽H5N1毒株的糖基化位点的氨基酸相同。结论2009年广东省新型甲型H1N1流感病毒NA基因与禽H5N1流感病毒高度同源，与NA抑制剂的特异性结合位点未发生变化。Objective To analyze the genetic characterization（evolution, antigenicity, enzyme activity sites and glycosylation sites）of the neuraminidase（NA）gene of the novel influenza virus A/ H1N1 in 2009 pandemic in Guangdong Province. Methods The viral RNA was extracted from 69 isolates of influenza virus A/HIN1 from patients in 2009 pandemic in Guangdong Province. NA gene fragments were amplified by reverse transcription polymerase chain reaction （RT-PCR） and sequenced. The other 52 NA gene sequences of influenza virus A in different years and different regions were retrieved from GenBank. The analysis of evolution and amino acid sequences were analyzed by MEGA 4.0 software. Results The homology of 2009 novel H1N1 influenza viruses in Guangdong and avian H5N1 influenza virus strains was high（〉85%）. The amino acid distributions of potential antigenic sites were identical. The enzyme activity sites of NA genes of all virus strains were strictly conserved, which had eight glyeosylation sites. But there were amino acid substitutions in 5 glycosylation sites, while it was identical with the 2001 avian HSN1 influenza virus. Conclusion The NA genes of 2009 novel H1N1 influenza viruses in Guangdong are high homologous with avian H5N1 influenza virus and the viral specific binding sites of neuraminidase inhibitor are not changed.

关 键 词：流感病毒A型 H1N1亚型 神经氨酸酶 进化分子 变异(遗传学) 

分 类 号：R686[医药卫生—骨科学]