丹参酮ⅡA-聚乳酸/羟基乙酸微球对兔肝动脉栓塞作用的研究  

The embolization effect of tanshinoneⅡA-polylactic acid/glycolic acid microspheres on the hepatic artery in experimental rabbits

作　　者：陈红宇[1,2] 李琦[1,2] 陈庆华[3] 赵泽华[1,2] 韩蕾[3] 王之[1,2] 徐嵩森[1,2] 王炎[1,2] 

机构地区：[1]上海中医药大学附属普陀医院 [2]上海中医药大学中西医结合肿瘤介入研究所,200062 [3]上海医药工业研究院

出　　处：《介入放射学杂志》2010年第12期977-981,共5页Journal of Interventional Radiology

基　　金：上海市重点学科资助项目(No.S30302);上海市卫生局青年基金资助项目(No.2009Y91)

摘　　要：目的观察经肝动脉注入丹参酮ⅡA-聚乳酸/羟基乙酸微球对兔肝动脉的栓塞作用。方法新西兰兔24只在DSA监视下经肝动脉注入丹参酮ⅡA微球,注入后10 min,1、3、7、14、21、30、42 d各取3只再次造影,观察肝动脉栓塞情况,并处死取肝、心、脾、肺、肾和胃组织,观察病理变化,同时作血常规和肝、肾功能检查。结果栓塞后10 min造影显示,肝动脉末梢血管消失。栓塞后第1、3、7、14、21、30 d造影,肝动脉末梢血管均未显影。栓塞后第42 d造影显示肝动脉末梢血管已显影。病理切片显示栓塞部位出现炎性及坏死。常规和生化结果:介入栓塞后白细胞出现一过性升高,第7 d恢复正常水平(P>0.05);AST、ALT均在栓塞后第3 d达到最高值,第7 d恢复正常水平(P>0.05)。结论丹参酮ⅡA微球具有良好的肝动脉末梢栓塞效果,栓塞时间在30～42 d,是一种理想的肿瘤介入栓塞剂。Objective To observe the embolization effect of tanshinoneⅡA-polylactic acid/glycolic acid microspheres（PLGA） on the hepatic artery in experimental rabbits.Methods Under DSA guidance,hepatic catheterization and angiography was performed in 24 New Zealand white rabbits.Via the catheter tanshinoneⅡA-PLGA microspheres was injected into the hepatic arteries.Each time at 10 minutes and on the 1 st,3 rd,7 th,14 th,21 st,30 th and 42 nd day after the treatment,every three rabbits were randomly selected for the re-examination with angiography to observe the embolization state of the hepatic arteries,then the animals were sacrificed and the liver,heart,spleen,lung kidney and stomach were removed and sent for pathologic exam.Simultaneously,the functional tests of liver and kidney as well as the routine blood tests were made.The results were evaluated.Results The peripheral micro-vessels of the hepatic artery vanished away at 10 minutes after embolization,and they remained un-visualized till the 30th day after embolization.On the 42nd day after embolization the peripheral micro-vessels of the hepatic artery could be visualized again.Pathologically,signs of inflammation and necrosis appeared in the occluded areas.Blood biochemical examination showed that there was a transient elevation of white blood cells after the procedure,which fell to normal level on the 7th day（P 0.05）.Both AST and ALT reached their peaks on the 3rd day,then they decreased gradually and returned to normal on the 7th day（P 0.05）.Conclusion TanshinoneⅡA-PLGA microspheres have better embolization effect on the hepatic arteries,this effect lasts for 30 to 42 days.Therefore,tanshinoneⅡA-PLGA microsphere is an ideal embolization agent for the treatment of neoplasm.

关 键 词：丹参酮ⅡA 微球 聚乳酸/羟基乙酸 栓塞 介入 

分 类 号：R285.5[医药卫生—中药学]