茄子空间诱变效应及其AFLP分析  

Induced Effects of Space Flight on Eggplant (Solanum melongena L.) and AFLP Analysis on Mutants

作　　者：孙保娟[1] 黎振兴[1] 罗少波[1] 李植良[1] 李艳艳[2] 

机构地区：[1]广东省农业科学院蔬菜研究所,广州510640 [2]华南农业大学生命科学院,广州510642

出　　处：《植物生理学报》2010年第12期1205-1210,共6页Plant Physiology Journal

基　　金：广东省基础条件建设项目(2009B060600004);广东省国际科技合作项目(2007A050100036;2008B050300006);广州市科技攻关重点项目(2006Z-E0061);广州市科技招标项目(GZCQC0902FG06017-02);现代农业产业技术体系建设专项资金;农业公益性行业科研专项经费项目(nyhyzx07-007-14);广东省农科院院长基金项目(201008)

摘　　要：为了研究茄子空间诱变效果,比较空间诱变系与原始对照间的分子水平差异,揭示空间诱变的分子效应,对3个不同茄子原始自交系(K_1、L_1、M_1)及其对应空间诱变系(K_2、L_2、M_2)生物学性状进行了比较研究,并利用AFLP分子标记技术进行了DNA指纹分析。结果表明,与原始自交系相比,3个空间诱变系的生育期、株型、果形均未发生明显变化,发生变化的性状是平均单果重和种子千粒重。诱变系M_2的平均单果重比其对照增加16.82%,差异极显著;种子千粒重有降低趋势,其中L_2比L_2显著降低了10.24%。48对AFLP选择性扩增引物在6个自交系和诱变系间扩增得到40条可以揭示诱变系与原始自交系间多态性的条带;部分目标片段测序结果经BLAST分析表明与已报道的高频突变相关DNA区域或蛋白同源性很高。可见,空间诱变技术能够引起茄子的遗传变异,而且这种变异是以DNA水平的变异为基础的。In order to study the mutagenic effects of space flight on eggplant（Solarium melongena）,the biological characteristics were compared and the molecular difference was analyzed by amplified fragment length polymorphism （AFLP） between 3 original lines（K_1,L_1 and M_1） and their corresponding mutant lines（K_2,L_2 and M_2）.The result showed that,the growth periods,plant types and fruit shapes of the three mutant lines had no significant difference compared with the original lines,whereas the average weight per fruit and seed weight per thousand were varied significantly.The average fruit weight of mutant line M_2 increased significantly by 16.67%than that of its original line M_1,and the seed weight per thousand of L_2 decreased significantly by 10.24%than that of L_1.40 polymorphic bands between original lines and mutant lines were amplified by 48 AFLP selective amplification primer combinations.By BLAST analysis,it was showed that the sequence of some target AFLP fragments were highly homologous to the reported DNA region or protein implicated in high frequency mutation.It revealed that space flight could create genetic variations in eggplant which were based on the variation of their DNAs.

关 键 词：茄子 空间诱变 AFLP标记 

分 类 号：S641.1[农业科学—蔬菜学]