LeWRKY1基因的克隆及分析  

Isolation and Analysis of Le WRKY1

作　　者：于涌鲲[1] 王丽芳[1,2] 杜希华[2] 赵福宽[1] 孙清鹏[1] 

机构地区：[1]北京农学院生物技术学院,北京102206 [2]山东师范大学生命科学学院,济南250014

出　　处：《植物生理学报》2010年第12期1225-1231,共7页Plant Physiology Journal

基　　金：北京市自然科学基金(5102015)

摘　　要：采用RT-PCR和RACE技术克隆了番茄LeWRKY1 cDNA(Genbank登录号为FJ654265)全长,用生物信息学的方法对其进行分析,预测其编码的蛋白的定位和功能。同时利用Northern杂交技术分析了JA(jasmonic acid)或SA(salicylic acid)刺激时LeWRKY1在野生型番茄和番茄JA不敏感突变体jail(JASMONATE-INSENSITIVE1)及番茄JA生物合成突变体spr2(prosystemin-mediated responses2)中的表达情况。LeWRKY1 cDNA序列全长1 740 bp,阅读框1 083 bp。生物信息学分析表明LeWRKY1含有1个WRKY结构域和1个C_2H_2型锌指结构。LeWRKY1可能是一种定位于细胞核的DNA结合蛋白,并可能具有转录调控、信号传导功能。表达分析推测LeWRKY1的表达是JA依赖而非SA依赖性的,且LeWRKY1的表达不依赖于生物体内JA的从头合成。The full length of LeWRKY1 cDNA（GenBank no.FJ654265） was cloned by RT-PCR and RACE approaches,and its cellular localization and function was deduced by bioinformatics methods.Using Northern blotting,LeWRKY1 expression in wild type tomato,JA insensitive tomato mutation jai1,and JA biosynthesis mutation spr2 were analyzed.The cloned LeWRKY1 cDNA was 1 740 bp with a 1 083-bp ORF.Bioinformatics analysis showed that LeWRKY1 might be a DNA binding protein located in the nucleus contained a WRKY domain and a C_2H_2 zinc finger motif,which might have the function of transcription,transcriptional regulation and signal transduction.Expression analysis indicated LeWRKY1 in tomato was JA dependent but SA independent, and LeWRKY1 was induced by JA,but it is independent on the de novo synthesis of JA.

关 键 词：番茄 LeWRKY1 茉莉酸 水杨酸 

分 类 号：Q943.2[生物学—植物学] S641.2[农业科学—蔬菜学]