人骨形成蛋白7修饰骨髓间充质干细胞复合仿生型支架材料的体内成骨研究  

In vio osteogenic potential of an implanted complex containing hBMP7-modified BMSCs and biomimetic composite scaffolds

作　　者：康焱[1] 康亮[2] 陈晓锋[3] 盛瑛义[1] 傅明[1] 袁振华 王迎军[3] 廖威明[1] 

机构地区：[1]中山大学附属第一医院骨科,广州510080 [2]中山大学附属第六医院 [3]华南理工大学材料科学与工程学院 [4]美国北卡罗莱娜大学教堂山分校药学院

出　　处：《中华生物医学工程杂志》2010年第5期421-425,共5页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金（30872615）;教育部高等学校博士点新教师基金（200805581170）;广东省自然科学基金（8151008901000137）

摘　　要：目的 应用携带人骨形成蛋白7（hBMP7）基因的兔骨髓间充质干细胞（BMSC）与仿生型生物玻璃-胶原-透明质酸-磷脂酰丝氨酸（BG-COL-HYA-PS）支架材料复合培养,植入兔桡骨缺损模型中观察其在体内成骨的能力.方法 携带hBMP7基因或增强型绿色荧光蛋白（EGFP）基因的2型重组腺相关病毒（rAAV2）载体在体外分别转染兔BMSC,再将转染后和未转染的兔BMSC分别与BG-COL-HYA-PS支架材料复合培养7 d后植入3组兔桡骨缺损模型,每组6只兔.各组在分别术后8周、12周通过大体标本观察、影像学、组织学等方法观察骨缺损的修复情况.以正常兔桡骨为对照组（n=3）,术后12周比较各组骨缺损修复组织生物力学差异.结果 rAAV2-hBMP7转染的兔BMSC与BG-COL-HYA-PS复合支架材料有良好的生物相容性,植入兔桡骨缺损模型内表现出明确的成骨能力和骨修复能力,而形成的新骨最大压力载荷值低于正常桡骨组织[（188.46±12.24）N比（203.25±19.29）N,P＜0.05].结论 用hBMP7修饰BMSC复合仿生型BG-COL-HYA-PS支架材料构建的组织工程骨具有较强的骨修复能力,但形成的新生骨组织与正常骨组织比较仍然有早期生物力学方面的不足.Objective To investigate the osteogenic potential in vivo of a cultured complex containing human bone morphogentic protein（hBMP）7-modified rabbit bone marrow mesenchymal stem cells （BMSCs） and bioglass-collagen-hyaluronic acid-phosphatidylserine （BG-COL-HYA-PS）, a biomimetic composite scaffolds, implanted in segmental radial defect in rabbit models. Methods Rabbit BMSCs were cultured and transfected with rAAV2-hBMP7 or rAAV2-EGFP plasmids respectively in vitro. The transfected or non-transfected BMSCs were then cultured with biomimetic BG-COL-HYA-PS scaffolds for 7 days, and implanted into the radial segmental defect in 3 groups of rabbits （n=6 each）. Eight and 12 weeks after implantation, gross inspection, imaging and histological studies were performed to observe the repair of bone defect. The biomechanics of repaired tissues in each group as compared with normal controls （n=3） was determined on week 12. Results Good biocompatibility, strong osteogenic potential and bone repairing was found for the complex of BG-COL-HYA-PS scaffolds and rAAV2-hBMP-7 transfected BMSCs after implanted in radial segmental defect of rabbit models. However, the strength of newly-formed rabbit radius was weaker than normal radius in terms of maximum pressure load [ （188.46± 12.24）N vs （203.25± 19.29）N, P〈0.05 ].Conclusion A gene modified tissue-engineering bone, constructed by BMSCs transfected with hBMP-7 gene and biomimetic BG- COL-HYA- PS composite scaffolds, shows better capacity for bone repairing.However, the newly- formed bone tissue appears biomechanically insufficient during the early phase compared with normal bone tissue.

关 键 词：骨形态发生蛋白质类 间质干细胞 骨替代材料 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]