探讨阴中求阳与阳中求阴对谷氨酸单钠大鼠下丘脑-垂体-肾上腺-胸腺轴的影响  

Effect of Regulation of Kidney Yin and Kidney Yang on Hypothalamus Pituitary Adrenal Thymus Axis in Monosodium L Glutamate Rats

作　　者：蔡定芳[1] 陈晓红[1] 刘彦芳[1] 李文伟 柏洁[1] 沈思钰 陈锡群 阮晴珉 

机构地区：[1]上海医科大学中西医结合研究所

出　　处：《中国中西医结合杂志》1999年第7期415-417,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金;卫生部基金;上海市卫生系统百人计划资助

摘　　要：目的：研究弓状核（ＡＲＣ）毁坏后下丘脑－垂体－肾上腺－胸腺（ＨＰＡＴ）轴的变化以及与肾阴肾阳的内在联系。方法：给出生后（第２、４、６、８、１０天）大鼠每天皮下注射左旋谷氨酸单钠（ＭＳＧ）４ｍｇ／ｇ损毁下丘脑ＡＲＣ，成年后分别以左归丸与右归饮灌胃（每天５ｇ／ｋｇ）。结果：ＭＳＧ大鼠下丘脑室旁核促肾上腺皮质激素释放激素（ＣＲＨ）阳性细胞及垂体前叶促肾上腺皮质激素（ＡＣＴＨ）分泌细胞数量明显多于生理盐水组，染色较深；肾上腺束状带紊乱不齐细胞数量增多，血窦扩张充血明显；胸腺萎缩，淋巴细胞增殖反应明显低于生理盐水组；血浆皮质酮及血浆ＡＣＴＨ和下丘脑ＣＲＨ等浓度高于生理盐水组。左归丸组上述病理生理变化得到明显改善，右归饮则无此作用。结论：ＨＰＡ轴功能亢进伴细胞免疫功能低下的病理生理过程可能属肾阴虚证范畴。Objective: To study the interelationship between hypothalamus pituitary adrenal thymus (HPAT) axis and Kidney Yang and Kidney Yin in arcuate nucleus destroyed rats. Methods: Monosodium glutamate (MSG) 4 mg/g body weight was given subcutaneously to neonatal rats at 2, 4, 6, 8 and 10th day after born to destroy the ARC, and Zuogui pill or Yougui pill (5g/kg daily) was given respectively by gastrogavage when the model rats entered adulthood. Results: Immunohistochemical staining showed that in the model group the number of corticotropin releasing hormone (CRH) positive neurons in hypothalamic paraventricular nucleus and anterior pituitary adrenotrophin (ACTH) positive secretory cells, which stained deeply, were more than those in the control group, the adrenal fasciculate zone disturbed with increased cells and obviously dilated sinusoid. The thymus atrophied with lymphocyte proliferation apparently lower, blood corticosterone, ACTH content and hypothalamic CRF level higher in the model than those in the control. Zuogui pill could improve above mentioned pathophysiologic changes effectively but Yougui pill could not. Conclusion: Process of pathophysiololgy of HPA axis hyperfunction accompanied cell mediated immunodeficiency may belong to the category of Kidney Yin Deficiency Syndrome.

关 键 词：左旋谷氨酸单钠 HPA 阳中求阴 阴中求阳 

分 类 号：R285.5[医药卫生—中药学] R2-031[医药卫生—中医学]