化学与药效学指标相结合改进痛经宝颗粒中挥发油提取工艺  

Innovation on Extract Process of Volatile Oil in Tongjingbao Granules by Chemical Index and Pharmacodynamics Index

作　　者：王绪颖[1] 陈彦[1] 张振海[1] 王丽静[1] 贾晓斌[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统重点实验室,国家中医药管理局中药释药系统重点研究室,南京210028

出　　处：《中国实验方剂学杂志》2011年第9期15-21,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省公益研究和服务(BM2007701);江苏省社会发展科技支撑计划(BE2010756)

摘　　要：目的:改进痛经宝颗粒中挥发油的提取工艺。方法:以藁本内酯、桂皮醛、木香烃内酯和去氢木香内酯的含量为化学指标改进痛经宝颗粒中当归、肉桂、木香挥发油的提取工艺,优选了挥发油超临界CO2提取的最佳条件,并采用小鼠痛经模型、大鼠子宫痉挛模型比较了工艺改进前后制剂的药效。结果:当归单独提取、肉桂、木香合并提取挥发油的方法都以超临界CO2萃取较佳,当归挥发油最佳萃取条件为压力25 MPa,温度40℃,萃取2 h;肉桂、木香挥发油最佳萃取条件为压力35MPa,温度55℃,萃取2 h。工艺改进后制剂的解痉作用与原制剂比较明显增加。结论:经过挥发油的工艺改进,提高了痛经宝制剂的疗效,且超临界CO2萃取当归、肉桂、木香挥发油的工艺简便可行。Objective：To innovate and optimize the extract process of volatile oil in Tongjingbao granules.Method： Ligustilide,cinnamaldehyde,costunolide and dehydro-α-curcumene were used as chemical index and together with mice dysmenorrheal model and rat uterine spasm model,the best extract process of volatile oil in Radix Angelicae Sinensis,Cortex Cinnamomi Cassiae and Radix Aucklandiae were optimized.It was investigated by orthogonal experiment to optimize the conditions and parameters to extract essential oil.Result： Both the best extract process of Radix Angelicae Sinensis（single）,Cortex Cinnamomi Cassiae and Radix Aucklandia（mix） were CO2 supercritical fluid extraction,and the spasmolysis effect of innovated process had significant increase than the original preparation.The optimal extraction conditions of the essential oil from Angelica sinensis using supercritical fluid extraction were： pressure 25 MPa,temperature 40 ℃,extraction time 2 hour,and 35 MPa,55 ℃ and 2 hour for essential oil from Cinnamomum cassia and Aucklandia lappa.Conclusion： The improvement of the extraction process of volatile oil enhanced the efficacy of Tongjingbao preparation,and the process was feasible and convenient.

关 键 词：挥发油 提取工艺 化学指标 药效学指标 痛经宝颗粒 

分 类 号：R283.6[医药卫生—中药学]