正交试验优选水提后丹参药渣中丹参酮Ⅱ_A的提取工艺  

Study on Extract Process of Tanshinone Ⅱ_A in Salvia miltiorrhiza

作　　者：刘常青[1] 何百寅[1] 冯峰[1] 谢友良[1,2] 蒋东旭[1,2] 赖小平[1,2] 

机构地区：[1]广州中医药大学新药开发研究中心,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808

出　　处：《中国实验方剂学杂志》2011年第9期32-34,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2009A030901001)

摘　　要：目的:探讨水提后丹参药渣中丹参酮ⅡA的最佳提取工艺条件。方法:以丹参酮ⅡA提取率为定量考察指标,采用L9(34)正交试验,考察了提取时间、提取次数、乙醇倍量及乙醇体积分数对丹参酮ⅡA提取率的影响。结果:丹参药渣中丹参酮ⅡA的最佳提取工艺条件为:8倍量90%乙醇浸泡30 min,回流提取2次(85℃),每次0.5 h,高效液相色谱法检测,水提后丹参药渣中丹参酮ⅡA的提取率约为91%。结论:优化的提取条件效率高,水提后丹参药渣中丹参酮ⅡA的含量较高,提示其可以作为一种丹参酮资源,具有开发利用的价值。Objective：To explore the best condition to extract tanshinone ⅡA in Salvia mihiorrhiza by ethano1.Method： The optimum condition was determined with L9（34） orthogonal experiment to arrange experiments with extract rate of tanshinone ⅡA as quantitative index.Result： The best extraction process condition was： 8 times of 90 percent alcohol immersed for 30 minutes,reflux extraction was two times with each time of 0.5 hour at 85 ℃.The HPLC result showed that the extraction rate of tanshinone ⅡA was about 91%.Conclusion： Optimized extraction process design is reasonable,reproducible and can increase the reuse of herbs.Salvia mihiorrhiza can be served as a kind of tanshinone resource,which has great value of development and utilization.

关 键 词：提取率 丹参酮ⅡA 水提后丹参药渣 正交试验 

分 类 号：R283.6[医药卫生—中药学]