藕节、藕节炭HPLC特征图谱研究  

HPLC Specific Chromatograms of Nelumbinis Rhizomatis Nodus and Nelumbinis Rhizomatis Nodus Charcoal

作　　者：单玲玲[1] 刘善新[2] 靳光乾[2] 梁瑞雪[2] 

机构地区：[1]山东中医药大学,济南250355 [2]山东省中医药研究院,济南250014

出　　处：《中国实验方剂学杂志》2011年第9期67-71,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家十一五科技支撑项目(2006BAI09B006-02)

摘　　要：目的:建立藕节、藕节炭HPLC特征图谱。方法:以3-表白桦脂酸为参照物,采用Lichrospher C18柱(4.6 mm×150mm,5μm),乙腈-0.2%磷酸为流动相梯度洗脱,柱温30℃,流速1 mL.min-1,检测波长203 nm,分析时间70 min。结果:藕节、藕节炭分别获得5,4个共有特征峰,同时标定了3-表白桦脂酸的位置。结论:该方法操作简便,重复性好,特征性强,为藕节、藕节炭的质量控制提供了新参考。Objective：To set up the HPLC specific chromatograms of Nelumbinis Rhizomatis Nodus andNelumbinis Rhizomatis Nodus charcoal.Method： The analysis was performed on Lichrospher C18column（4.6 mm × 150 mm,5 μm） with a mobile phase consisting of acetonitrile-0.2% phosphoric acid by gradient elution at a column temperature of 30 ℃;the flow rate was 1.0 mL.min-1,the detection wavelength was at 203 nm and the analytic time was 70 min.Result： Five specific peaks were identified in chromatograms of Nelumbinis Rhizomatis Nodus and four specific peaks were identified in chromatograms of Nelumbinis Rhizomatis Nodus charcoal by 3-epibetulinic acid as reference substance.Conclusion： The method is simple and reliable with a good reproducibility and can be used for the quality control of Nelumbinis Rhizomatis Nodus and Nelumbinis Rhizomatis Nodus Charcoal.

关 键 词：藕节 藕节炭 高效液相色谱法 特征图谱 

分 类 号：R284.1[医药卫生—中药学]