泥胡菜黄酮类化学成分研究  

Flavonoids from Hemistepta lyrata

作　　者：邬秀娟[1,2] 王黎[1,2] 管丽娜[1] 张英华[1] 董方言[1] 王威[1] 

机构地区：[1]吉林省中医药科学院植物化学研究所,长春130012 [2]延边大学药学院,吉林延吉133000

出　　处：《中国实验方剂学杂志》2011年第9期107-110,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技支撑计划项目(2007BAI38B05)

摘　　要：目的:研究泥湖菜乙醇提取物氯仿和乙酸乙酯分离部位化学成分。方法:采用硅胶柱色谱,ODS柱色谱,Sephadex LH-20柱色谱和制备液相色谱等手段进行化学成分分离,运用理化性质和波谱数据(MS,1 H-NMR,13 C-NMR,HMBC等)鉴定化合物结构。结果:从泥湖菜乙醇提取物氯仿和乙酸乙酯分离部位分离得到8个黄酮类化合物,分别鉴定为金合欢素(1),玄参黄酮(2),芹菜素(3),山萘酚(4),山萘酚-3-O-β-D-吡喃葡萄糖苷(5),山萘酚-7-O-β-D-吡喃葡萄糖苷(6),山萘酚-3-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖苷(7),金合欢素-7-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖苷(8)。结论:化合物5为首次从该属植物中分离得到。Objective：To investigate the chemical constituents from the chloroform and ethyl acetate fractions of ethanol extract from Hemistepta lyrata.Method： The compounds were isolated by silica gel chromatography,ODS chromatography,Sephadex LH-20 chromatography,and preparative HPLC,and their structures were elucidated on the basis of chemical and spectroscopic methods,including MS,1D and 2D NMR spectral techniques.Result：Eight flavonoids were isolated from the chloroform and ethyl acetate fractions of ethanol extract from H.lyrata,and were identified as acacetin（1）,cirsimaritin（2）,apigenin（3）,kaempferol（4）,kaempferol-3-O-β-D-glucopyranoside（5）,kaempferol-3-O-β-D-glucopyranoside（6）,kaempferol-3-O-α-L-rhamnopyranosyl-（1 →6）-β-D-glucopyranoside（7）,and acacetin-7-O-α-L-rhamnopyranosyl-（1 →6）-β-D-glucopyranoside（8）.Conclusion： This is the first report of the isolation of 5 from Hemistepta genus.

关 键 词：泥湖菜 黄酮 化学成分 

分 类 号：R282.71[医药卫生—中药学]