不同干燥方法对祛湿化瘀方防治实验性脂肪肝药效学的影响  

Effects of Different Drying Methods of Qushi Huayu Decoction on Pharmacodynamics of Experimental Fatty Liver

作　　者：冯琴[1] 张钰泉[2] 张宁[2] 李红山 李琦[1] 许丽莉[1] 彭景华[1] 刘平[1] 胡义扬[1] 

机构地区：[1]上海中医药大学附属曙光医院,肝肾疾病证候教育部重点研究室,上海市高校中医内科学E-研究院,上海201203 [2]上海中医药大学科技实验中心,上海201203

出　　处：《中国实验方剂学杂志》2011年第9期137-141,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(30672635);国家自然基金青年基金项目(81001486);上海市教委创新团队项目(第一期)

摘　　要：目的:探讨不同干燥方法对"祛湿化瘀方"防治实验性脂肪肝药效学的影响。方法:祛湿化瘀方中5味药物分别醇提、水提醇沉后合并,制备成生药含量为0.93 g.mL-1的药液,再按不同干燥方法制得浸膏、70℃真空负压干燥药物、40℃真空负压干燥药物、冷冻干燥药物、喷雾干燥药物。采用Wistar雄性大鼠,以四氯化碳(CCl4)复合高脂低蛋白饮食制备大鼠脂肪肝模型,造模2周后,将造模大鼠随机分为模型组、未干燥组、浸膏组、70℃真空负压干燥组、40℃真空负压干燥组、冷冻干燥组、喷雾干燥组,每组灌以相应药物2周。取材后检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷氨酰转移酶(GGT)活性、肝组织甘油三酯(TG)、游离脂肪酸(FFA)的含量变化。并对各个药物样品进行指纹图谱分析。结果:与正常组相比,模型组ALT,AST,GGT活性,肝组织TG,FFA含量显著升高。未干燥组与模型组相比,血清ALT,AST,GGT及肝组织TG,FFA显著下降;浸膏组与模型组相比,血清GGT,ALT,肝组织TG,FFA显著下降;70℃和40℃真空干燥组除血清ALT活性显著降低外,其余指标与模型组相比均无显著差异;冷冻干燥组除肝组织TG显著低于模型组,其余指标与模型组相比均无显著差异;喷雾干燥组除血清GGT显著低于模型组,其余指标与模型组相比均无显著差异;未干燥组的综合疗效优于其他各组。运用指纹图谱对各组药物进行分析,色谱图有相应的谱峰信息综合表达有差异。结论:"袪湿化瘀方"经不同干燥方法后对实验性脂肪肝药效均有所下降,不同方法干燥后部分有效物质丢失,可能是导致药效差异的主要原因。Objective：To discuss the effects of different drying methods of Qushi Huayu decoction on thepharmacodynamics of experimental fatty liver.Method： Qushi Huayu decoction was prepared（0.93 g crude drug.mL-1）,then been concentrated to paste,and dried powder through 70 ℃ vacuum drying,40 ℃ vacuum drying,frozen drying and spray drying.Wistar male rats were used to establish fatty liver model by carbon tetrachloride（CCl4） subcutaneous injection for 4 weeks（twice per week） along with high-fat and low-protein diet for 2 weeks.After 2 weeks,the rats were respectively divided into model group,non-drying group,paste group,70 ℃ vacuum drying group,40 ℃ vacuum drying group,frozen drying group and spray drying group randomly and given corresponding drug.The rats were killed after treatment for 2 weeks,then activity of serum ALT,AST,GGT,and content of liver TG,FFA were detected.The different drugs were analyzed through fingerprint analysis.Result： Activity of serum ALT,AST,GGT and content of liver TG and FFA were all increased in the model group.Compared with the model group,ALT,AST,GGT,TG and FFA of the non-drying group were significantly decreased.The paste group had good effect on ALT,GGT,TG and FFA.Compared with the model group,activity of serum ALT were significantly decreased in 70 ℃ vacuum drying and 40 ℃ vacuum drying group,content of liver TG were significantly decreased in frozen drying group,activity of serum GGT were significantly decreased in spray drying group.Fingerprint Analysis showed the chromatogram map of each drug were different.Conclusion： Different drying methods have negative effects for Qushi Huayu decoction on the pharmacodynamics of experimental fatty liver.Some effective material may be dropped after drying.It needs further research.

关 键 词：祛湿化瘀方 干燥方法 实验性脂肪肝 药效学 指纹图谱分析 

分 类 号：R285.5[医药卫生—中药学]