不同溶剂对鸡血藤提取物总黄酮含量及抗肿瘤活性的影响  

Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from Spatholobus suberectus

作　　者：程悦[1] 王志宇[2] 王冬梅[1] 刘浩文[1] 陈建萍[2] 

机构地区：[1]中山大学药学院生药学与天然药物化学实验室,广州510006 [2]香港大学中医药学院

出　　处：《中国实验方剂学杂志》2011年第9期142-145,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨不同溶剂对鸡血藤提取物中的总黄酮含量和抗肿瘤活性的影响。方法:分别用不同浓度的乙醇水溶液、丙酮水溶液作为提取溶剂,利用回流法、超声提取法制备鸡血藤提取物;采用四甲基噻唑蓝(MTT)法测定提取物对结肠癌HT-29细胞增殖的抑制活性;采用分光光度法测定提取物中总黄酮含量;采用HPLC法测定提取物中芒柄花素含量。结果:60%乙醇回流60 min所得提取物总黄酮含量最高并且对HT-29细胞增殖的抑制活性最强。结论:鸡血藤抗肿瘤活性提取物的制备以60%乙醇回流提取为佳。Objective：To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method.Method： Using ethanol or acetone aqueous solution as the solvent,different extracts were prepared from S.suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC,respectively.Result： The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract.Conclusion： Using 60% ethanol as extraction solvent,refluxing as extraction method,obtained extract from S.suberectus showed highest anti-tumor activity on HT-29 cancer cell.

关 键 词：鸡血藤 抗肿瘤 总黄酮 芒柄花素 细胞毒性 提取工艺 

分 类 号：R285.5[医药卫生—中药学]