龙葵多糖提取分离工艺研究  

Study on the Technology for Extracting and Separating Polysaccharide from Solanum Nigrum L.

作　　者：王淑萍[1] 郭艳平[1] 

机构地区：[1]长春工程学院理学院,吉林长春130012

出　　处：《信阳师范学院学报（自然科学版）》2011年第2期258-260,共3页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：长春工程学院自然科学基金(3200705)

摘　　要：采用水浴法设计三因素三水平正交试验,对龙葵多糖提取工艺进行研究;采用乙醇沉淀法设计三因素三水平正交试验对其分离工艺进行探索.正交试验结果表明:水浴法提取中三因素对龙葵多糖提取的影响顺序为:提取温度>料液比>提取时间,最佳提取工艺为:料液比1∶10,提取时间30 m in,提取温度80℃;乙醇沉淀法分离龙葵多糖中三因素的影响顺序为:乙醇浓度>沉淀时间>乙醇加入量,最佳分离工艺为:乙醇浓度90%,沉淀时间8 h,乙醇加入量3倍体积,这些条件的确定为龙葵的大规模开发和应用奠定了基础.The orthogonal experiment with three factors and three levels was designed for the nigrum polysaccharide extraction techonology by water bath method. The experimental result of orthogonal experiment showed that the influence factors of water bath is in the order as ： extract temperature 〉 ratio of solid to liquid 〉 extract time. The optimal extraction condition is obtained with ratio of solid to liquid of 1：10 at 80 ℃ and 30 minutes for three times. The influence factors of ethanol precipitation were in the order as ： the concentration of ethanol 〉 precipitation time 〉 ethanol volume. The optimal separation condition is obtained with 90% ethanol solution and precipitation for 8 hours . All of these conditions set the foundation for large - sale development and application of solanum nigrum L.

关 键 词：龙葵多糖 提取工艺 分离工艺 

分 类 号：Q81[生物学—生物工程]