猪乙型脑炎病毒3种灭活疫苗的制备及免疫效果比较  

Preparation and Comparison of Three Different Adjuvant Inactivated Vaccines of Japanese Encephalitis Virus from Swine

作　　者：乔进平[1] 赵明秋[1] 张学涛[1] 李银光[1] 李姗姗[1] 刘薇[1] 陈金顶[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2011年第2期85-88,共4页Journal of South China Agricultural University

基　　金：农业公益性行业科研专项(200803015);广东省自然科学基金创新团队项目(5200638);教育部"长江学者和创新团队发展计划"创新团队项目(TRT0723)

摘　　要：以GZ0409-31株乙脑强毒株作为抗原与白油佐剂、蜂胶佐剂、603纳米佐剂制备灭活疫苗,并对疫苗做了无菌检验和安全检验,灭活疫苗接种小鼠后均未出现不良反应,安全性良好.将制备的乙脑灭活疫苗免疫小鼠,并且与市售乙脑弱毒活苗作对比.结果显示,白油佐剂灭活苗组和603纳米佐剂灭活苗组在第90 d抗体效价还维持在较高的水平,同期的弱毒活苗组抗体较低;弱毒活苗组IFNγ-含量较灭活苗组高,其中603纳米佐剂灭活苗组IFNγ-含量较其他灭活苗组高.用乙脑病毒强毒对免疫小鼠进行感染,白油佐剂灭活苗和603纳米佐剂灭活苗对小鼠的保护率均为100%(10/10),蜂胶佐剂灭活苗对小鼠的保护率为70%(7/10),对照组市售乙脑弱毒活苗对小鼠的保护率为100%(10/10).表明制备白油佐剂灭活苗和603纳米佐剂灭活苗对小鼠的保护率高,无排毒散毒危险.The oil-emulsion,propolis and 603 nanometer inactivated vaccines of Japanese encephalitis virus GZ0409-31 strain from swine were prepared and compared by sterility and safety test.Mice immunized with these inactivated vaccines displayed effective immune protection that testifies their safety.The groups of mice immunized with the oil-emulsion and 603 nanometer inactivated vaccines had higher antibody titer until 90 days post inoculation whereas the group of mice immunized with attenuated Japanese encephalitis vaccine had lower antibody titer.In addition the level of IFN-γ was higher in the group of mice immunized with attenuated Japanese encephalitis vaccine than those groups with inactivated Japanese encephalitis vaccines.The group of mice immunized with 603 nanometer inactivated Japanese encephalitis vaccines expressed higher level of IFN-γ than those other inactivated Japanese encephalitis vaccines.Mouse immunized with the oil-emulsion,propolis and 603 nanometer inactivated vaccines could be protected from the infection of GZ0409-31 Japanese encephalitis virus with 100% protection rate in case of oil-emulsion and 603 nanometer inactivated vaccines immunized groups but remained 70% in propolis inactivated vaccines immunized groups.The attenuated Japanese encephalitis vaccine also provided 100%（10/10）protection rate.The oil propolis and 603 nanometer inactivated GZ0409-31 Japanese encephalitis vaccines were prepared and found to be safe and effective.

关 键 词：乙脑病毒 灭活疫苗 佐剂 免疫效果 

分 类 号：S855.3[农业科学—临床兽医学]