链霉菌Q0102b菌株培养条件优化  

Optimum Cultural Conditions for Streptomyces sp.Q0102b

作　　者：王再强[1] 何月秋[1] 陈贤[1] 夏金娥[1] 周红[1] 杨大庆[1] 

机构地区：[1]云南农业大学农业生物多样性与病虫害控制教育部重点实验室,云南昆明650201

出　　处：《华南农业大学学报》2011年第2期116-118,共3页Journal of South China Agricultural University

基　　金：科技部国际合作项目(2009DFA32360);国家自然科学基金(30660007);云南省教育厅科学研究基金(08C0083);昆明市校企合作项目(09H130301)

摘　　要：Q0102b是一株具有广泛抑菌作用的链霉菌菌株.本文研究了7种碳源和4种氮源对该菌株生长量及抑菌活性的影响.当玉米淀粉为碳源、酵母浸出汁为氮源时,Q0102b培养液对燕麦镰刀菌Fusarium avenaceum(Corda et Fr.)Sacc.的抑制作用最强.正交试验结果表明,该菌株高抑菌活性的最佳培养条件为玉米淀粉40 g/L、酵母浸出汁7g/L、氯化钠10 g/L、pH 8.0,培养液不稀释,不加碳酸钙,28℃,装液量100 mL,75 r/min频率下振荡培养12 d.Streptomyces sp.Q0102b has a wide fungal repression spectrum.The cultural conditions including 7 carbon and 4 nitrogen sources were studied for its growth amount and antifungal activity.The corn starch and yeast extract used as carbon and nitrogen sources showed the strongest repression on Fusarium avenaceum.Based on orthogonal test,the optimized cultural condition for Q0102b＇s strong antifungal activity is 40 g/L corn starch,7 g/L yeast extract,10 g/L NaCl,packed 100 mL in 250 mL flask at pH 8.0 and 28 ℃ for 12-day incubation in a shaker at 75 r/min.

关 键 词：放线菌 培养条件 优化 

分 类 号：S432.43[农业科学—植物病理学]