不同深度冻土微生物数量特征及其与土壤理化性质的关系  

Quantitative Characters of Microorganisms in Permafrost at Different Depths and Their Relation to Soil Physicochemical Properties

作　　者：王鹭[1] 董小培 张威[3] 章高森[3] 刘光琇[3] 冯虎元[1] 

机构地区：[1]兰州大学生命科学学院,甘肃兰州730000 [2]巩义市第二高级中学,河南郑州451200 [3]中国科学院寒区旱区环境与工程研究所沙漠与沙漠化重点实验室,甘肃兰州730000

出　　处：《冰川冻土》2011年第2期436-441,共6页Journal of Glaciology and Geocryology

基　　金：国家自然科学基金项目(307703293080015440971034);中国博士后科学基金项目(20080430794)资助

摘　　要：分别以乌鲁木齐河源区不同层位深坑冻土样品为研究材料,利用荧光显微计数技术、寡营养恢复培养技术等研究了微生物数量特征及其与土壤理化参数的关联度.结果表明:可培养细菌数量与冻土深度、土壤含水量、总C和总N含量都成显著的正相关,与pH值呈显著负相关.而且,可培养细菌数及其在细胞总数中所占比值随冻土深度的增加而下降,表明深坑样冻土中可培养细菌数量与冻土的理化性质和其沉积年代密切相关.The microorganisms in permafrost could live in cold environment through the coadapted physicochemical process,which exists between microorganisms in permafrost and permafrost environment.In this paper,the relation between microbial number and soil physicochemical properties of permafrost at different depths at the headwaters of the ürümqi River is analyzed by using fluorescence microscopy counting and oligo-culture techniques.Totally,20 samples from a 200-cm permafrost core were used as study materials.It is found that the number of cultivable bacteria has a significant positive correlation with soil water content,total carbon and total nitrogen concentrations,and a significant negative correlation with pH value.Meanwhile,the ratio of cultivable bacteria to total cell number decreases with depth.The results demonstrate that the number of cultivable bacteria in permafrost is closely correlated with soil physicochemical properties and depositional age.

关 键 词：冻土微生物 可培养细菌 理化性质 相关性 

分 类 号：Q938.13[生物学—微生物学]