电针对胶原诱导性关节炎大鼠外周血与关节液中TNF-α、VEGF的影响  

Electroacupuncture on TNF-α and VEGF in Peripheral Blood and Synovial Fluid in Collagen-induced Arthritis Rat Models

作　　者：高洁[1] 欧阳八四[1] 叶晓群[1] 车建丽[2] 张音[1] 胡天燕[3] 杨海洲[1] 

机构地区：[1]苏州市中医医院,江苏215009 [2]苏州大学附属第一医院,江苏215007 [3]苏州大学附属儿童医院,江苏215003

出　　处：《上海针灸杂志》2011年第4期272-274,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：苏州市科技发展计划资助项目(SS0528)

摘　　要：目的观察CIA大鼠造模成功后外周血和关节液中TNF-α、VEGF的改变,并且观测电针治疗后这些指标的变化。方法取样后测定CIA大鼠外周血和关节液中TNF-α、VEGF的含量,并与正常的大鼠样本进行比较;对CIA大鼠按疗程进行治疗,测定治疗后的这些指标,对指标进行治疗前后比较、与模型组比较。结果 CIA大鼠外周血和关节液中TNF-α、VEGF的含量高于正常组(P<0.05);电针治疗后这些指标有了显著改善(P<0.05),与模型组比较差异有统计学意义(P<0.05)。结论电针能有效降低CIA大鼠外周血和关节液中TNF-α、VEGF的含量,这些致炎性因子的降低对阻止CIA大鼠关节滑膜细胞浸润、破骨细胞激活,抑制病变发展有非常重要的意义。Objective To observe the changes of TNF-α and VEGF in peripheral blood and synovial fluid in rats models of collagen-induced arthritis（CIA）,and their changes after electroacupuncture.Methods The samples were obtained to determine the concentrations of TNF-α and VEGF in peripheral blood and synovial fluid in CIA rats and normal rats,and then made a comparison.The involved indexes were measured before and after electroacupuncture treatment and then compared them with each other as well as with that of the model group.Result The concentrations of TNF-α and VEGF in peripheral blood and synovial fluid in CIA rats were higher than in the normal,and the differences were statistically significant（P0.05）;the indexes had marked improvement after receiving electroacupuncture（P0.05）,and the difference was significant（P0.05）.Conclusion Electroacupuncture effectively lower the concentrations of TNF-α and VEGF in peripheral blood and synovial fluid in CIA rats,and the reduction of the proinflammatory cytokines plays a crucial role in preventing synovial cell infiltration and activation of osteoclasts in CIA rats,as well as in inhibiting the development of disease.

关 键 词：电针 大鼠 胶原诱导性关节炎 肿瘤坏死因子Α 血管内皮生长因子 

分 类 号：R2-03[医药卫生—中医学]