黑线仓鼠LHR部分序列的克隆与分析  

Cloning and Sequence Analysis of LHR in Cricetulus Barabensis

作　　者：谢海燕[1] 薛慧良[1] 徐金会[1] 王哲[1] 徐来祥[1] 

机构地区：[1]曲阜师范大学生命科学学院,山东省曲阜市273165

出　　处：《曲阜师范大学学报（自然科学版）》2011年第2期79-83,共5页Journal of Qufu Normal University(Natural Science)

基　　金：国家"973计划"项目(No.2007CB109104);国家自然科学基金项目(No.31070332);山东省高校科技计划项目(J09LC18)

摘　　要：以大鼠、小鼠、金仓鼠(Mesocricetus auratus)的同源保守序列设计了黑线仓鼠(Cricetulus barabensis)黄体生成素受体基因(LHR)的引物,经PCR扩增得到了154 bp的目的产物,通过Blast证实该片段是黑线仓鼠的LHR序列.该段序列处于第11外显子的5’端,编码51个氨基酸,包含部分铰链区和第一个跨膜α螺旋(TM1)区.疏水性氨基酸所占比例相当高,其中缬氨酸、异亮氨酸各占9.80%,亮氨酸为19.61%,通过疏水性分析,TM1区具有典型的α螺旋特征.与大鼠、小鼠、金仓鼠等物种同源比对,核苷酸和氨基酸的同源性分别达到了83%-98%,82%-100%.α螺旋跨膜区的氨基酸同源性超过82%具有很强的保守性,但在26,41,44,48位点上仍然表现出种属之间的差异性.该研究用PCR的方法克隆出了黑线仓鼠LHR的部分序列,并对其进行了同源性分析,为今后黑线仓鼠种群繁殖机制、种群扩散模式等的研究奠定一定理论基础.In order to obtain the gene sequence of luteinizing hormone receptor（LHR） of Striped hamster（Cricetulus barabensis）,the primer was designed according to the homology sequence of golden hamsters（Mesocricetus auratus）,mice（Mus musculus） and rats（Rattus norvegicus）,a 154 bp LHR sequence had been obtained by PCR reaction.This fragment sequence localized at the 11th exon of 5＇ and encoded 51 amino acid including partial hinge region and the first transmembrane helical segment（TM1）.The hydrophobic value indicates typically α-helix in TM1 and the proportion of Val,Ile,Leu were 9.80%,9.80%,19.61% respectively.Alignment of LHR fragment sequence with that of other species,the nucleotide and amino acid homology were 83%-98%,82%-100% respectively.Although amino acid homology of TM1 was conserved and up to 82%,there is significant distinction on sites 26,41,44,48 between rodents and other species.Furthermore,it can provide theoretical basis for its further application,such as the mechanism of population reproductive and the pattern of population dispersion

关 键 词：黑线仓鼠 LHR TM1 克隆分析 

分 类 号：Q349[生物学—遗传学]