巨大芽胞杆菌培养条件的研究  

Culture Conditions for Bacillus megaterium

作　　者：陈秋红[1,2] 孙梅[1,2] 史济筠 邹益东 施大林[1,2] 胡凌 张维娜[1,2] 匡群 谢骏[1] 何义进[1] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,江苏无锡214081 [2]江苏省苏微微生物研究有限公司,江苏无锡214063 [3]宜兴天石饲料有限公司,江苏宜兴214258

出　　处：《畜牧与饲料科学》2011年第2期3-7,共5页Animal Husbandry and Feed Science

基　　金：农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金项目(BZ2009-21);江苏省科技计划项目(科技支撑计划-农业部分;BE2009371);江苏省无锡市科技支撑(农业攻关)项目(CMEN0904)

摘　　要：[目的]为了提高巨大芽胞杆菌的芽胞形成率及芽胞数量,为巨大芽胞杆菌芽胞制剂的产业化生产提供理论依据。[方法]通过单因子试验及正交试验对巨大芽胞杆菌JD-2发酵培养基和培养条件进行了研究。[结果]最适培养基组成为:玉米淀粉10.0g/L,蛋白胨5.0g/L,酵母膏5.0g/L,NaCl5.0g/L,CaCO31.0g/L,MgSO4·7H2O0.5g/L。确定最佳培养条件为:接种后起始芽胞浓度控制在106CFU/mL,初始pH值为7.0,培养温度为30℃,200r/min摇瓶培养,250mL三角瓶中最适装液体积为25mL。在15L自动发酵罐中扩大培养,控制溶氧在30%以上,培养22h,菌体浓度可达3.50×109CFU/mL,芽胞数量可达3.40×109CFU/mL,芽胞率达97.2%。[结论]试验获得的最佳培养条件可进一步应用于生产实际。[Objective] To increase sporulation rate and spore concentration and offer the theoretical foundation for industrial production of Bacillus megaterium preparation. [Method] Fermentation culture medium and culture conditions of Bacillus megaterium JD-2 were studied by single factor test and orthogonal test. [Result] The optimum medium formula was composed of 10.0 g/L corn starch, 5.0 g/L peptone, 5.0 g/L yeast extract, 5.0 g/L NaCl, 1.0 g/L CaCO3 and 0.5 g/L MgSO4·7H2O. The optimum culture conditions are as follows： initial spore concentration, 10^6 CFU/mL; initial pH value, 7.0; culture temperature, 30 °C; shaking speed, 200 r/min; and volume, 25 mL medium in a 250 mL flask. When enlarge fermentation was performed in a 15 L auto fermentor with dissolved oxygen level less than 30%, cell concentration reached 3.50×10^9 CFU/mL, spore concentration reached 3.40×10^9 CFU/mL and sporulation rate reached 97.2% after 22 h culture. [Conclusion] The optimal culture conditions can be applied in practical production.

关 键 词：巨大芽胞杆菌 单因子试验 正交试验 培养条件 

分 类 号：S852.616[农业科学—基础兽医学]