高效液相色谱法分析测定大鼠肠系膜血管网灌流液中皮质醇、醛固酮方法的研究  

Cortisol and aldosterone in perfused rat mesenteric arteries measured by high performance liquid chromatography

作　　者：叶绿[1] 王煊[1] 赵清[2] 周俊岭[2] 张永生[3] 

机构地区：[1]第一军医大学珠江医院中心实验室,广州510282 [2]第一军医大学中心实验室,广州510515 [3]第一军医大学南方医院心内科,广州510515

出　　处：《第一军医大学学报》1999年第3期263-265,共3页Journal of First Military Medical University

摘　　要：采用高效液相色谱（HPLC）测定高血压模型大鼠肠系膜血管网灌流过程中灌流液皮质醇、醛固酮的含量，为定量分析血管分泌皮质醇和醛固酮机制提供可靠的量化依据，建立用HPLC分离检测皮质醇和醛固酮的方法。结果表明该方法准确、快速、稳定，检测灵敏度可达到50ng／L，仪器系统重复测定变异系数为2．32％，标准曲线线性分析相关系数r均大于99％，平均回收率均大于90％。To quantitatively analyze the secretion ofcortisol and aldosterone in perfused rat mesenteric emeries. High performanceliquid chromatography (HPLC) was used for circuit peffosion of the hypertensive rat mesenteric artery. The method for analyzingand measuring cortisol and aldoStCrone in peffoSate by HPLC was established. The resultS showed that the method is accurate, rapidand sensitive, and the sensitivity was 50 ng/L. The variation coefficient for the equipment system was 2.32%. The correlationcoefficient for the standard curve was greater than 99%, and the recovery rate was higher than 90%.

关 键 词：HPLC 血管网灌流液 皮质醇 醛固酮 高血压 

分 类 号：R544.104[医药卫生—心血管疾病] R446[医药卫生—内科学]