PCR-DGGE检测GCG-R基因第二外显子  

Study a missense mutation in the glucagon receptor gene of exon 2 in NIDDM by PCR DGGE

作　　者：王玉明[1] 段勇[1] 宋滇平[1] 赵淮[1] 刘华[1] 杨惠英 

机构地区：[1]昆明医学院第一附属医院检验科

出　　处：《临床检验杂志》1999年第4期209-211,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：糖尿病是一种与遗传有着密切联系，并危害人类健康的常见病和多发病。最近，国外有人发现胰高血糖素受体（ＧＣＧ－Ｒ）基因上的一个错义突变与常见型非胰岛素依赖性糖尿病（ＮＩＤＤＭ）高度相关。本文应用聚合酶链反应一变性梯度凝胶电泳技术（ＰＣＲ－ＤＧＧＥ）对２００例无亲缘关系中国昆明地区汉族人的ＧＣＧ－Ｒ基因第二外显子研究结果表明，所有研究对象均不存在错义突变。Diabete mellitus is a generically and recurrent disease which hazard the people's health,with relatively to heterogene.Lastest,oversea researchers had found that there is one missense mutation high relative with NIDDM patients in GCG R gene. It was observed to 160 the Han peoples by PCR DGGE in Kunming region of China,results showed that there were no missense mutation in all samples,so indicated the G1y40Ser missense mutation have not relation with NIDDM in Kunming region of China.

关 键 词：胰高血糖素受体 糖尿病 NIDDM PCR-DGGE 

分 类 号：R587.102[医药卫生—内分泌]