棉花枯萎病菌AFLP分子标记体系的建立及初步应用  

Establishment and Application of AFLP Analysis System for Fusarium oxysporum f.sp.vasinfectum

作　　者：王晓光[1,2] 郭庆港[2] 么奕清 李社增[2] 鹿秀云[2] 李宝庆[2] 马平[2] 

机构地区：[1]河北农业大学植物保护学院,河北保定071000 [2]河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000 [3]河北省邯郸市农业局,河北邯郸056001

出　　处：《棉花学报》2011年第2期147-153,共7页Cotton Science

基　　金：现代农业产业技术体系专项资金

摘　　要：建立并优化了基于HaeIII/PstI酶切的棉花枯萎病菌AFLP分析的最佳反应体系,并从32对引物组合中筛选出适合该反应体系的引物组合3对。应用该技术对存在于我国的棉花枯萎病菌3号、7号和8号生理小种的标准菌株进行分析,结果表明该技术能够有效地将这3个标准菌株区分开;通过该体系对来自我国4个省的20个棉花枯萎病菌菌株进行分析,其聚类分析结果表明在0.96 Dice相似系数水平上,20个棉花枯萎病菌菌株与棉花枯萎病菌7号生理小种聚在一个类群内,属于7号生理小种,并且小种种群内不同菌株间存在一定程度的遗传多样性。An amplified fragment length polymorphism（AFLP） analysis system was constructed by using HaeIII/PstI digested DNA to analyze different isolates of Fusarium oxysporum f.sp.vasinfectum.Three primer combinations were selected from 32 primer pairs and employed in the optimized AFLP system.Three reference strains representing physiological races 3,7 and 8 of F.oxysporum f.sp.vasinfectum from China were characterized by the optimized AFLP system.Cluster analysis using data generated by AFLP makers showed the system could clearly separate all three F.oxysporum f.sp.vasinfectum races.AFLP analysis of 20 F.oxysporum f.sp.vasinfectum isolates from four provinces categorized all isolates into the race 7 group in high level of Dice similarity coefficients（0.96）,and the genetic diversities were observed among different isolates in the same physiological group.

关 键 词：棉花枯萎病菌 AFLP 分子标记 优化 遗传多样性 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]