利用根箱法解析转双价(Bt+CpTI)基因棉花对土壤微生物数量及细菌多样性的影响  

Effects of Transgenic Bt +CpTI Cotton on the Soil Microbial Amount and Bacterial Diversity by Rhizobox Method

作　　者：娜布其[1,2] 红雨[1] 杨殿林[2] 赵建宁[2] 李刚[2] 娜日苏[1,2] 刘玲[2,3] 

机构地区：[1]内蒙古师范大学,呼和浩特010022 [2]农业部环境保护科研监测所/农业部转基因生物生态环境安全监督检验测试中心(天津)中国农业科学院武清转基因生物农田生态系统影响野外科学观测试验站,天津300191 [3]甘肃农业大学,兰州730070

出　　处：《棉花学报》2011年第2期160-166,共7页Cotton Science

基　　金：转基因生物新品种培育重大专项课题(2008ZX08012-004);中央级公益性科研院所基本科研业务费专项资金(农业部环境保护科研监测所)(2009-ZJN-11)

摘　　要：利用根箱法对棉花根部土壤进行分区采集,并采用传统平板培养和DGGE克隆测序技术相结合的方法,对转双价基因棉和常规棉3个生长时期(播种后40 d、50 d和60 d)不同根区土壤细菌、放线菌和真菌数量及细菌多样性进行对比分析。平板培养结果表明,与常规棉相比,转双价基因棉S1根区土壤细菌、放线菌的数量显著降低,而S2根区土壤细菌、放线菌、真菌和S3根区土壤细菌、真菌的数量显著增加(P<0.05);DGGE图谱分析表明,在棉花生长的3个时期内,转双价基因棉和常规棉根区土壤细菌多样性指数、均匀度和条带数均无显著差异(P>0.05),2种棉花处理间有着很强的相似性,不受转双价基因棉种植的影响。转双价基因棉种植仅改变了根区土壤可培养微生物数量,对细菌多样性无显著影响。In this study,three-room rhizobox method was used to collect soil samples from different zones of cotton root.Traditional culture-depending methods and DGGE-cloning sequencing were used to investigate the different soil microbial（bacteria,actinomycetes,fungi） amount and bacterial diversity between transgenic Bt ＋CpTI cotton and conventional cotton in three growing stages（40 d,50 d and 60 d）.The results of traditional culture-depending method showed that the amount of soil bacteria and actinomycetes decreased significantly in S1 zone of transgenic Bt ＋CpTI cotton,but the amount of three microorganisms in S2 zone and the amount of bacteria and fungi in S3 zone of transgenic Bt ＋CpTI cotton increased significantly,compared to the conventional cotton.DGGE fingerprinting indicated that the Shannon-Wiener index,evenness and the number of bands had no significant difference between transgenic Bt ＋CpTI cotton and conventional cotton in three growing stages.There was high similarity between the two cotton treatments and it was not affected by the planting of transgenic Bt ＋CpTI cotton.The planting of transgenic Bt ＋CpTI cotton only affected the amount of microorganisms and had no significant effect on the bacterial diversity.

关 键 词：转双价基因棉 土壤微生物 根箱法 细菌多样性 

分 类 号：S562[农业科学—作物学]