新疆某地区维吾尔族HIV高危人群趋化因子受体5多态性与艾滋病的关系  

RESEARCH ON THE RELATIONSHIP BETWEEN AIDS AND THE POLYMORPHISM OF HIV INFECTION-RELATED GENE CCR5 IN HIV HIGHLY-INFECTED UYGUR POPULATION IN A CERTAIN AREA OF XINJIANG UYGUR AUTONOMOUS REGION

作　　者：唐慧[1,2] 郭淑霞[1] 赵蓉莉 张景玉[1] 谭晓华[1] 

机构地区：[1]新疆石河子大学医学院,石河子832002 [2]石河子大学科技处 [3]石河子市疾病预防控制中心

出　　处：《现代预防医学》2011年第9期1699-1701,1712,共4页Modern Preventive Medicine

基　　金：国家自然科学基金项目(30700687);新疆兵团科技攻关项目(医药卫生专项)(05GG36)

摘　　要：[目的]检测HIV-1感染辅助受体CCR5Δ32、CCR5m303a在新疆维吾尔族HIV高危人群中基因突变频率,探讨CCR5Δ32、CCR5m303a基因突变与新疆维族HIV高危人群感染艾滋病的关系。[方法]以470名维吾尔族HIV高危人群(经Western印迹法确诊HIV阳性246人,HIV阴性224人)为研究对象,抽提全血基因组DNA,多重PCR扩增,多重LDR反应,用测序仪检测CCR5Δ32和CCR5m303a的突变频率。[结果]检测结果为:CCR5Δ32等位基因突变频率为4.47%,纯合子突变2例(HIV阳性、阴性者各1例),杂合子突变38例(HIV阳性24例,HIV阴性14例)。CCR5m303a等位基因突变频率为2.76%,杂合子突变26例(HIV阳性22例,HIV阴性4例),无纯合子突变。CCR5Δ32基因突变在维族HIV高危人群,HIV阳性和HIV阴性人群中分布差异无统计学意义(P﹥0.05)。CCR5m303a基因突变在维族HIV高危人群HIV阳性组和HIV阴性组分布差异有统计学意义(P﹤0.05)(OR=5.4,OR95%CI为1.83～15.93);在不同性别的维族HIV高危人群中突变频率差异有统计学意义(P﹤0.05)。并且在HIV阳性人群中有3人同时具有CCR5Δ32杂合子突变和CCR5m303a的杂合子突变。[结论](1)CCR5Δ32基因突变在高危人群HIV阳性组和HIV阴性组中分布差异无统计学意义,提示:CCR5Δ32基因突变与维族HIV高危人群艾滋病感染无关。(2)维族HIV高危人群CCR5m303a基因突变在HIV阳性组和HIV阴性组中分布差异有统计学意义,CCR5m303a基因突变可能是HIV高危人群艾滋病感染的有害因素,发生CCR5m303a基因突变的人群感染艾滋病的危险性是未发生突变的人群的5.4倍。[Objective]To do research on the distribution characteristics of infection-aiding receptor CCR5 in HIV-1 in HIV highly-infected Uygur population of Xinjiang.At the same time to investigate the relationship between CCR5 Δ32,CCR5m303a genetic mutation frequency and HIV highly-infected Uygur population of Xinjiang.[Methods]The research took 470 samples of the Uygur population as its subjects,including 246 HIV positives and 224 negatives after being tested by Western Impression Method.Then we detested the mutation frequency of CCR5 Δ32 and CCR5m303a.[Results]The result showed that the mutation frequency for CCR5Δ32 was 4.47% and there were 2 samples of homozygote mutation（one was HIV positive and the other was HIV negative） and 38 samples of heterozygote mutation（24 samples were HIV positive and 4 samples were negative） .The result also showed that the mutation frequency for CCR5m303a was 2.76% and there were 26 samples of heterozygote mutation（22 samples were HIV positive and 4 were negative） and no example of homozygote mutation.There was no statistical meaning for CCR5Δ32 mutation in distribution difference in HIV highly-infected Uygur population of Xinjiang（P﹥0.05） ,either positive or negative.However,There was statistical meaning for CCR5m303a mutation in HIV highly-infected Uygur population of Xinjiang（P﹤0.05）（OR=5.4,OR95%CI was from 1.83 to 15.93） and it was also statistically meaningful in mutation frequency difference with different genders of these HIV population（P﹤0.05） .Finally there were 3 positive samples who had CCR5Δ32 heterozygote mutation and CCR5m303a heterozygote mutation simultaneously.[Conclusion]（1） There is no statistical significance in the distribution difference of CCR5Δ32 mutation in the HIV infection group and the contrast group,suggesting that probably CCR5Δ32 mutation is not the protecting factor in Uygur HIV infection population.（2） There is statistical significance in the distribution difference of CCR5m303a mutation in these two groups

关 键 词：CCR5Δ32 CCR5m303a 基因多态性 HIV高危人群 维吾尔族 

分 类 号：R512.91[医药卫生—内科学]