小麦品系中梁93444的抗条锈性遗传分析与分子作图  

Genetic analysis and molecular mapping of stripe rust resistance gene in wheat line Zhongliang93444

作　　者：王岭岭[1] 侯冬媛[1] 王文立[1] 周新力[1] 宋建荣[2] 井金学[1] 

机构地区：[1]西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,杨凌712100 [2]甘肃省天水市农业科学研究所,天水741001

出　　处：《植物保护学报》2011年第2期109-115,共7页Journal of Plant Protection

基　　金：高等学校学科创新引智计划(B07049);小麦条锈病菌毒性变异与条锈病综合治理研究示范(200903035-02)

摘　　要：为明确抗锈品种中梁93444抗条锈基因及遗传特点,用CYR30、CYR31、CYR32对该品种、铭贤169及杂交组合进行遗传分析,用SSR技术对分离家系F3-3进行PCR扩增和电泳分析。结果显示,中梁93444对CYR30、CYR31的抗病性均由1对显性和1对隐性基因控制,对CYR32由2对显性互补基因控制;F3-3分离家系对CYR32的抗病性由1对显性基因控制,该基因暂命名为Yr93444。对F3-3分离群体进行SSR标记,建立了与该基因连锁的8个标记Xgwm122、Xwmc702、Xwmc644、Xwmc794、Xgwm328、Xwmc455、Xgwm372、Xwmc819,遗传距离分别为38.1、30.7、22.9、15.6、10.0、6.9、3.5和2.8 cM。应用SSR标记、中国春及缺四体将Yr93444定位于2AL上。系谱分析和SSR分子标记检测表明,该基因是来自中间偃麦草的新抗条锈基因。用与该基因紧密连锁的SSR标记Xgwm372和Xwmc819检测中梁品种和黄淮麦区主栽品种,发现89%中梁品种含该抗病基因,而86%黄淮麦区主栽品种不含该抗病基因,表明该基因应用潜力很大。Zhongliang93444 is highly resistant to three rusts of wheat.To identify and map the gene for stripe rust resistance,CYR30,CYR31 and CYR32 were selected to test Zhongliang93444,Mingxian169 and generations of Zhongling93444 crossed with Mingxian169.SSR technology was used to map the gene resistant to CYR32 based on F3-3 line.The results showed that the resistance in Zhongliang93444 to CYR30 and CYR31 were controlled by one dominant and one recessive gene,respectively.The resistance to CYR32,however,was controlled by two complementary dominant genes.The resistance of the family separation F3-3 of Zhongliang93444/Mingxian169 to CYR32 was controlled by one dominant gene which was temporarily designated as Yr93444.Eight SSR markers,Xgwm122,Xwmc702,Xwmc644,Xwmc794,Xgwm328,Xwmc455,Xgwm372,and Xwmc819,were linked to Yr93444,and the genetic distances were 38.1,30.7,22.9,15.6,10.0,6.9,3.5 and 2.8 cM,respectively.SSR markers,21 nulli-tetrasomic and Chinese Spring lines located the gene on wheat chromosome 2AL.The pedigree analysis and molecular maker results indicated the origin of the gene was from Elytrigia intermedium.Yr93444 was detected in 89% of Zhongliang wheat cultivars by Xgwm372 and Xwmc819 as PCR primers,and was not detected in 86% of the 42 tested wheat cultivars from Huanghuai area with Xgwm372 and Xwmc819 as PCR primers.The results were useful for breeding resistance cultivars to stripe rust.

关 键 词：中梁93444 抗条锈性 遗传分析 分子标记 

分 类 号：S512.1[农业科学—作物学]