荧光光谱法研究抗癌新药吡柔比星与DNA的相互作用  

STUDIES ON THE STYLE OF INTERACTION OF PIRARUBICIN WITH DNA BY FLUORESCENCE SPECTRA

作　　者：胡敏[1,2] 杨欣[1,2] 晋卫军 曹祉祥[1,2] 沈国励 俞汝勤[1,2] 

机构地区：[1]湖南大学化学化工学院 [2]化学计量学与化学传感器技术研究所

出　　处：《药学学报》1999年第8期608-612,共5页Acta Pharmaceutica Sinica

基　　金：高校博士点基金;湖南省科委基金

摘　　要：目的：研究抗癌新药吡柔比星（ＴＨＰ）与ＤＮＡ的作用方式。方法：利用荧光光谱法和吸收光谱法，以ＴＨＰ为荧光探针研究测定ＤＮＡ。结果：ＴＨＰ与ＤＮＡ的结合常数为１６６×１０５Ｌ·ｍｏｌ－１，结合位点数为２６ｂａｓｅｐａｉｒｓ。此外，利用ＤＮＡ对ＴＨＰ的荧光猝灭作用研究了对ＤＮＡ的分析应用。ＤＮＡ的检出限为０９１μｇ·ｍＬ－１，线性范围为０９１～９０μｇ·ｍＬ－１和９０μｇ·ｍＬ－１～２６μｇ·ｍＬ－１。结论：ＴＨＰ的蒽醌环与ＤＮＡ的碱基对发生嵌入作用，而ＴＨＰ的含糖侧链与ＤＮＡ有小沟槽作用。AIM: To study the characteristics of the interaction of pirarubicin (THP) with DNA. METHODS: Study and determination of DNA with THP as a fluorescence probe was presented by absorption and fluorescence spectra. RESULTS: The binding constant of the interaction between THP and DNA is 1 66×10 5 L·mol -1 , and the binding site number is about 2 6 base pairs. The limit of detection is 0 91 μg·mL -1 and the linear ranges are 0 91～9 0 μg·mL -1 and 9 0～26 μg·mL -1 respectively for the determination of DNA. CONCLUSION: It indicates that the anthracycline moiety of THP intercalates the stacked base pairs of DNA, which is the major controlling factor. In addition, the complex sugar structure of THP may be bound to the minor groove of DNA double helix.

关 键 词：荧光光谱法 抗癌药 吡柔比星 嵌入作用 DNA 

分 类 号：R979.1[医药卫生—药品]