慢性疲劳综合征患者外周血淋巴细胞亚群及CD25^+调节性T细胞表达的研究  

Expression of Lymphocyte Subsets and CD25^+ Regulative T Cells in Peripheral Blood of Patients with Chronic Fatigue Syndrome

作　　者：刘定华[1] 刘占东[2] 李永哲[3] 张海莹[3] 胡朝军[3] 张拥波[2] 王得新[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,北京100730 [2]首都医科大学附属北京友谊医院神经内科,北京100050 [3]中国医学科学院北京协和医学院北京协和医院风湿免疫科,北京100730

出　　处：《标记免疫分析与临床》2011年第2期93-96,共4页Labeled Immunoassays and Clinical Medicine

基　　金：国家高技术研究发展技划(863计划)基金资助项目(2006AA02Z436)

摘　　要：研究慢性疲劳综合征(CFS)患者外周血淋巴细胞亚群及CD25+调节性T细胞的表达情况。使用流式细胞仪检测84例CFS患者(CFS组)、50例健康体检者(健康对照组)外周血淋巴细胞亚群(T细胞、CD4+T细胞、CD8+T细胞、B细胞、NK细胞)及CD25+调节性T细胞的表达情况。结果显示,CFS组与健康对照组的T细胞、CD8+T细胞百分率以及CD4+/CD8+比值无显著差别(P>0.05);而CFS组NK细胞、CD4+T细胞及CD25+调节性T细胞百分率显著增高,B细胞百分率显著降低(P<0.05)。结论:慢性疲劳综合征患者外周血淋巴细胞各亚群比例异常,提示其免疫功能失衡,而CD25+T调节性细胞可能在该病进程中有重要意义。To investigate the expression of lymphocyte subsets and CD25＋ regulative T cells in peripheral blood of patients with chronic fatigue syndrome（CFS）.Eighty-four CFS patients and fifty healthy volunteers were enrolled in this study.Their peripheral blood was sampled,and the lymphocyte subsets and CD25＋ regulative T cells were measured by flow cytometry.The results showed that the NK cells,CD4＋T cells and CD25＋ regulative T cells in CFS patients were higher and B cells were lower than that of healthy controls（P0.05）,while there was no significant difference between CFS patients and healthy controls with respect to the percentage of T cells and CD8＋T cells and CD4＋/CD8＋ ratio（P0.05）.The results indicated that CD25＋ regulative T cell might play an important role in CFS-related immunological abnormality,and be involved in the progress of CFS.

关 键 词：慢性疲劳综合征 淋巴细胞 CD25+调节性T细胞 流式细胞术 

分 类 号：R446.6[医药卫生—诊断学]