激素和变温处理对百合鳞片扦插繁殖的影响  

Effects of Plant Regulators and Alternating Temperature Treatment on Propagation during Lily Scale Cutting

作　　者：孙红梅[1,2] 安晶[1] 王春夏[1] 谢佳[1] 陶承光[2] 

机构地区：[1]沈阳农业大学园艺学院辽宁省设施园艺重点实验室,沈阳110866 [2]辽宁省农业科学院,沈阳110161

出　　处：《林业科学》2011年第3期39-45,共7页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30972023);中国博士后科学基金项目(20090451280);沈阳农业大学拔尖人才基金

摘　　要：以亚洲百合品种‘精粹’为试材,探讨GA3和IBA以及不同培养温度对鳞片扦插繁殖的影响。结果表明:低浓度IBA处理(IBA-80mg·L-1-1h)的繁殖系数较大、小鳞茎形成的整齐度较高,IBA处理可在较短时间内促使小鳞茎膨大;若培养时间充分,GA3处理更有利于形成个体较大的鳞茎。5℃低温预处理加速IBA处理母鳞片淀粉的降解和总可溶性糖的利用,进一步提高IBA处理的繁殖系数、平均级数和小鳞茎直径;但5℃低温预处理与GA3处理结合,抑制母鳞片中淀粉的降解以及小鳞茎对可溶性糖的吸收利用,致使小鳞茎生长缓慢。本试验条件下IBA-100mg·L-11h结合5℃低温预处理为提高繁殖系数和整齐度的最佳处理;GA3-100mg·L-1-2h结合25℃恒温培养最有利于小鳞茎的发育膨大,为获得优良个体的最佳处理。Effects of GA3,IBA as well as different cultivating temperature on scale cutting propagation were studied in Lilium Asiatic hybrid ‘Elite’.The results indicated that low concentration of IBA（IBA-80 mg·L-1-1 h） was better for improving propagation coefficient and produced higher uniformity of bulblets.IBA treatment promoted enlargement of bulblets within a short period.However,when the culture duration was long enough,GA3 was better for producing larger bulblets.With low temperature （5 ℃）pretreatment IBA accelerated the degradation of starch and the utilization of soluble sugar in the mother scales,further improved scale propagation coefficient and average grade as well as single bulblet diameter.But GA3 with the low temperature pretreatment inhibited the starch degradation in the mother scales and the utilization of soluble sugar in the bulblets,resulting in the bulblets grew slowly.Under the condition of this experiment,IBA-100 mg·L-1-1 h with low temperature both 5 ℃ pretreatment was the best treatment for improving the propagation cofficient and uniformity of bulblets,and GA3-100 mg·L-1-2 h with even 25 ℃ culture was most conductive to expansion of bulblets when cultured in a constant temperature of 25 ℃ ,which was the best treatment to obtain the excellent individuals.

关 键 词：百合 鳞片扦插 植物生长调节剂 变温处理 淀粉降解 

分 类 号：S718.43[农业科学—林学]