罗布麻叶总黄酮提取物促进小鼠抗抑郁基因CREB、BDNF的表达  

Accelerating Expression of Antidepressant Effect Associate-gene CREB and BDNF of Flavonoid Extract Content from Apocynum venetum Leaves

作　　者：郑梅竹[1,2] 刘春明[1] 丛大力[3] 时东方[1] 张语迟[1] 吴桂梅[1] 

机构地区：[1]长春师范学院中心实验室,长春130032 [2]吉林大学畜牧兽医学院,长春130062 [3]长春师范学院化学学院,长春130032

出　　处：《基因组学与应用生物学》2011年第2期184-189,共6页Genomics and Applied Biology

基　　金：国家自然科学基金资助项目(30970299);吉林省科技厅重点项目(20090936;吉林省教育厅项目([2009]D206)共同资助

摘　　要：罗布麻是中国药典收录的传统中药,本研究提取它的主要有效成分罗布麻总黄酮,采用经典抗抑郁评价模型小鼠强迫游泳实验对罗布麻叶总黄酮进行抗抑郁活性评价。试验中给小鼠罗布麻叶总黄酮提取物25mg/kg、50mg/kg和100mg/kg后,观察罗布麻叶总黄酮对小鼠强迫游泳不动时间的影响,结果显示罗布麻总黄酮提取物能显著缩短小鼠强迫游泳不动时间(P<0.05),药效与氟西汀相似,表明罗布麻具有明显的抗抑郁活性。在此基础上,应用皮质酮损伤的PC12细胞模型,采用荧光相对定量RT-PCR法检测罗布麻对皮质酮损伤的PC12细胞中脑内脑源性神经营养因子(BDNF)和环磷酸腺苷反应元件结合蛋白(CREB)表达水平的影响。结果表明,皮质酮处理后PC12细胞中BDNF、CREB基因的表达量最低,罗布麻处理后,表达量显著增加,较处理前增加了近十倍,且呈剂量依赖性。本研究结果提示罗布麻抗抑郁机制可能是通过(AC-cAMP-CREB)信号通路促进BDNF、CREB的基因表达而发挥的。Apocynum venetum was a traditional drug which was included by Chinese Pharmacopoeia.We extracted the major active component of Apocynum venetum-flavonoid and investigated its antidepressant effect and corresponding mechanisms based on depression animal models.The antidepressant effect and possible mechanism of flavonoid extract from Apocynum venetum leaves （AV-extract） were observed in forced swimming test of mice,administration of AV extract （25mg/kg,50mg/kg and 100mg/kg,ip） reduced the duration time of immobility significantly （P0.05） suggested that Apocynum venetum have outstanding effect of antidepression,and the drug action of it is similar with fluoxetine.To further search for the antidepressant mechanism of Apocynum venetum,corticosterone damaged PC12 cell was used as a model,meanwhile Taqman quantitative testing technique was used to test the expression of the brain-derived neurotrophic factor and cAMP response element binding protein of PC12 cells in the presence of corticosterone （0.01mmol/L） and flavonoid extract from Apocynum venetum leaves （25μg/mL,50 μg/mL and 100μg/mL） for 48 h.The result showed that the expression level of BDNF,CREB was reduced significantly after treatmented by corticosterone only,but was elevated significantly by Apocynum venetum treatment which was up-regulated approximately 10 times with dose-dependment corresponding to only corticosterone treated.The result showed that the possible mechanism of Apocynum venetum maybe related to elevation the expression of BDNF,CREB through the signal path AC-cAMP-CREB.

关 键 词：罗布麻叶总黄酮提取物 皮质酮 PC12细胞 脑源性神经营养因子 CAMP反应元件结合蛋白 

分 类 号：S563.7[农业科学—作物学] S865.13