疏水修饰聚阳离子高分子SP-Chol在COS-7细胞中转染和毒性的研究  

Study on Transfection and Cytotoxicity of Hydrophobically Modified Cationic Polymer SP-Chol in COS-7 Cells

作　　者：李晖[1] 刘一浓[1] 王菲[1] 魏蓓蓓[1] 周毅[1] 杜子秀[1] 吴飞[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《现代生物医学进展》2011年第7期1205-1207,共3页Progress in Modern Biomedicine

基　　金：国家"重大新药创制"科技专项(2009ZX09310-007:针对生物技术药物的相关给药技术平台)

摘　　要：目的:研究以精胺为单体,以乙二醇二氯甲酸酯作为连接剂,以胆固醇氯甲酸酯作为疏水基团连接剂合成的疏水修饰聚阳离子高分子SP-Chol对非洲绿猴肾癌细胞COS-7的转染活性和细胞毒性的影响。方法:以荧光素酶质粒为报告基因,研究SP-Chol与DNA的复合物在COS-7细胞的转染活性,用MTT方法研究SP-Chol对COS-7细胞的毒性。结果:COS-7细胞实验显示,SP-Chol具有低于PEI 25kDa的细胞毒性,同时也具有高效输送DNA的能力。结论:SP-Chol是一种新型的高效、低毒,在基因治疗领域有潜在应用价值的非病毒基因输送载体。Objective：To investigate the transfection efficiency and cell cytotoxicity of hydrophobically modified cationic polymer SP-Chol which is systhesized by using spermine as monomer,ethylenebis（chloroformate） as coupling agent and cholesterol chlorofor-mate as hydrophobic coupling agent.Methods：Luciferase plasmid was used as the reporter gene to investigate transfection efficiency on COS-7 cells.Cytotoxicity was detected by MTT method.Results：COS-7 cells transfection and cytotoxicity results suggested that SP-Chol was less cytotoxic than PEI 25kDa,and had a high transfection efficiency.Conclusion：SP-Chol is a highly efficient and less tox-ic non-viral gene delivery vector which has potential application in gene therapy.

关 键 词：疏水修饰 精胺 聚阳离子高分子 转染 毒性 

分 类 号：Q813[生物学—生物工程] Q813