基于钙黄绿素-铜(Ⅱ)荧光体系测定乙酰半胱氨酸  

Fluorimetric Determination of Acetylcysteine Based on Calcein-Copper(Ⅱ) System

作　　者：杨晓红[1] 景蓓蓓[1] 张胜海[1] 答敏[1] 张萍[1] 朱依娜[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,陕西西安710062

出　　处：《现代生物医学进展》2011年第7期1355-1358,共4页Progress in Modern Biomedicine

摘　　要：目的:基于钙黄绿素-铜(Ⅱ)荧光体系测定乙酰半胱氨酸。方法:在pH=8.0的Na2HPO.412H2O-KH2PO4缓冲液中,以492 nm为激发波长,520 nm为发射波长测定乙酰半胱氨酸溶液的荧光强度。结果:在pH=8.0的Na2HPO.412H2O-KH2PO4缓冲液中,二价铜离子与钙黄绿素配位引起荧光猝灭。由于乙酰半胱氨酸中巯基上的硫离子与Cu2+的亲和力很强,可从钙黄绿素-铜(Ⅱ)的络合物中夺取铜离子而使钙黄绿素游离出来,从而使体系的荧光得以恢复,并且荧光恢复的程度与加入乙酰半胱氨酸的量在一定范围内成线性。结论:建立了一种测定乙酰半胱氨酸的荧光分析新方法,该方法的线性范围为6.0 10-6～1.4 10-5 mol/L,检出限为4.010-6 mol/L。Objective：Fluorimetric Determination of Acetylcysteine in Calcein-Copper（Ⅱ） System.Methods：In pH=8.0 of KH2PO4-Na2HPO4·12H2O buffer solution,determinating the fluorescence intensity of acetylcysteine solutions with 492nm as excita-tion wavelength and 520nm as emission wavelength.Rusults：In pH=8.0 of KH2PO4-Na2HPO4·12H2O buffer solution,copper（Ⅱ） can coordinate with calcein,and cause the fluorescence intensity of calcein solution decreased.Because the appetency between copper（Ⅱ） and acetylcysteine was very strong,it can seize copper ions from calcein-copper（Ⅱ） complex and make calcein free,thus make the fluores-cence recovered,and the recovery degree of its fluorescence was linear related to the concentration of acetylcysteine added to the solution.Conclusions：Hereby we establish a new method of measuring acetylcysteine in the range of 6.0 10-6~1.4 10-5 mol/L.The detection limit was 4.0×10-6 mol/L.The relative standard deviation was 0.91 % for the 11 replicate determinations of 1.0×10-5 mol/L acetylcysteine solution.This method was applied to the determination of acetylcysteine in pharmaceutical preparations.

关 键 词：钙黄绿素 荧光 乙酰半胱氨酸 

分 类 号：Q503[生物学—生物化学]