基于钙黄绿素的荧光分光光度法测定谷胱甘肽  

Determination of Glutathione by Fluorescence Spectroscopy Method Based on Calcein-Copper(Ⅱ) System

作　　者：景蓓蓓[1] 杨晓红[1] 张胜海[1] 答敏[1] 张萍[1] 朱依娜[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,陕西西安710062

出　　处：《现代生物医学进展》2011年第7期1359-1362,共4页Progress in Modern Biomedicine

摘　　要：目的:基于钙黄绿素的荧光分光光度法建立一种测定谷胱甘肽的新方法。方法:在pH=8.0介质中,铜(Ⅱ)与钙黄绿素配位引起荧光猝灭,由于谷胱甘肽与铜(Ⅱ)的亲和力很强,可从钙黄绿素-铜离子的络合物中夺取铜离子而使钙黄绿素游离出来使荧光强度得以恢复,荧光恢复的程度与加入谷胱甘肽的浓度在一定浓度范围内成线性。结果:据此建立了一种测定谷胱甘肽的新方法,该方法的线性范围为2.0×10-6～1.4×10-5mol.L-1,F=8.1964C+196.43,检测限为1.0×10-6mol/L。结论:用此法测定谷胱甘肽简便快速,灵敏度高。Objective：To establish a new method of measuring reduced glutathione by calcein copper（Ⅱ）system.Methods：In pH=8.0 buffer solution,Copper（Ⅱ） can coordinate with calcein,and cause the fluorescence intensity of calcein solution decreased.Because of the affinity between Copper（Ⅱ） and reduced glutathione is very strong,so it can seize copper ions from calcein-Copper（Ⅱ） complex and make calcein free,thus make the fluorescence recovered,and the recovery degree of its fluorescence was linear related to the concentration of reduced glutathione added into the solution.Results：Hereby established a new method of measuring reduced glutathione in the range of with 2.0×10-6~1.4×10-5 mol·L-1,F=8.1964C＋ 196.43,a detect limit of 1.0×10-6mol/L.Conclusion：This method of measuring reduced glutathione is simple,quick and sensitive.

关 键 词：谷胱甘肽 荧光分光光度法 钙黄绿素 

分 类 号：Q503[生物学—生物化学]