二甲苯胺噻嗪对大鼠镇痛和麻醉的ED_(50)及时效的测定  

Determination of Analgesia and Anesthesia ED_(50) Values and Time effection Relationship of Xylazine on rats

作　　者：艾永兴[1] 李东郊[1] 田慧英[1] 闫章年[1] 

机构地区：[1]解放军农牧大学军事兽医研究所,长春130062

出　　处：《畜牧兽医杂志》1999年第4期1-4,共4页Journal of Animal Science and Veterinary Medicine

基　　金：国家自然科学基金

摘　　要：大鼠腹腔注射二甲苯胺噻嗪（ｘｙｌａｚｉｎｅ）后，采用序贯法（ＳｅｑｕｅｎｔｉａｌＡｎａｌｙｓｉｓ）测定对大鼠镇痛和麻醉的半数有效量（Ｍｅｄｉａｎ Ｅｆｆｅｃｔｉｖｅ Ｄｏｓｅ，ＥＤ５０ ）。结果，镇痛的ＥＤ５０值１５．５２４ ｍ ｇ／ｋｇ 麻醉的ＥＤ５０ 值为４５．０９６ ｍ ｇ／ｋｇ，在增加一个公比（ｒ镇痛＝ １．５０７，ｒ麻醉＝ １．１４３）的基础上，我们取２５．０ ｍ ｇ／ｋｇ 和５５ ｍ ｇ／ｋｇ用于镇痛和麻醉实验，结果表明，镇痛组大鼠在给药后１５、３０、４５、６０ ｍ ｉｎ 时甩尾反射潜伏期（ＴＥＬ）升高４０％ 以上，与生理盐水组比较差异极显著（ｐ ＜ ０．０１），麻醉组大鼠的翻正反射消失率达１００％ 。We determined the analgesia and anesthesia ED 50 of xylazine after intraperitoneal injection in rats with sequential analysis. The effectiveness of xylazine analgesia and anesthesia was assessed by tail flick latency(TFL) and the disappearance of righting reflex. The results were that the analgesia ED 50 was 15.524mg/kg and anesthesia ED 50 was 55mg/kg. We used the dosage, 25.0 mg/kg and 55 mg/kg, over ED 50 one common multiple in the formal research and determined the time effection relationship of xylazine in the two dosage to decided whether the dosages were optional. The results showed that the TFL was increased markedly (over 40%, p < 0.01)15、30、45、60 minute after and the percent of righting reflex two dosage can satisfy the further experimental requirements.

关 键 词：二甲苯胺噻嗪 序贯法 镇痛 麻醉 兽用药 

分 类 号：S859.791[农业科学—临床兽医学]