金铁锁HPLC多组分指纹图谱研究  

Study on the HPLC Finger-prints of RADIX PSAMMOSILENES

作　　者：项世军[1] 贾宪生[2] 胡成刚[2] 

机构地区：[1]贵阳药用植物园,贵州贵阳550002 [2]贵阳中医学院药学系,贵州贵阳550002

出　　处：《安徽农业科学》2011年第10期5770-5772,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]采用HPLC建立1、3、5年生金铁锁(RADIX PSAMMOSILENES)的多组分指纹性图谱,为金铁锁的鉴别、质量控制提供全面、合理和科学的依据。[方法]采用Agilent Zorbax SB C-18(150.0 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸溶液(B)为流动相进行梯度洗脱,洗脱程序为0～5 min,15%～20%A;5～45 min,20%～45%A;45～75 min,45%～85%A;75～80 min,85%A。柱温25℃,流速0.5 ml/min,检测波长为210 nm。根据最佳色谱条件获得3个年限金铁锁药材多组分指纹性图谱,以金铁锁提取液经薄层分离得较纯色谱峰为参照峰。[结果]建立了金铁锁药材的3个年限的多组分指纹性方法,该方法简单、重现性好,可用于金铁锁1、3、5年品种的鉴别。[结论]该研究可为金铁锁的鉴别和质量控制全面、合理和科学的依据。[Object] To establish HPLC finger-prints of one year,three-year and five-year RADIX PSAMMOSILENES,and provide reasonable basis for the identification and quality control of RADIX PSAMMOSILENES.[Method] Agilent Zorbax SB C18 column（150.0 mm×4.6 mm,5μm）,mobile phase of acetonitrile（A） and 0.1% phosphoric acid（B）,gradient elution： 0-15 min,15%-20% A;5-45 min,20%-45% A;45-75 min,45%-85% A： 75-80 min,85% A.Column temperature of 25 ℃,flow rate of 0.5 ml/min,detective wavelength of 210 nm.3 HPLC finger-prints were obtained under the optimum HPLC conditions,and the chromatographic peak of purified RADIX PSAMMOSILENES was as reference.[Result] HPLC finger-print method was simple and reproducible,which was suitable for the identification of one year,three-year and five-year RADIX PSAMMOSILENES.[Conclusion] This study would provide reasonable and scientific basis for the identification and quality control of RADIX PSAMMOSILENES.

关 键 词：金铁锁 HPLC 多组分指纹性图谱 

分 类 号：S567[农业科学—中草药栽培]