成都样品中粘细菌的分离纯化  

Isolation and Purification of Myxobacteria in Chengdu

作　　者：刘冰花[1] 杨林[1] 冯礼福[1] 何柳兴[1] 

机构地区：[1]成都大学医护学院,四川成都610015

出　　处：《安徽农业科学》2011年第10期5868-5869,5911,共3页Journal of Anhui Agricultural Sciences

基　　金：四川省教育厅资助科研项目(08zb092);成都大学校基金(2009XJZ16;2010XJZ32)

摘　　要：[目的]为进一步研究粘细菌中生物活性物质奠定基础。[方法]从成都各区县采集35份水样、土样、兔粪、羊粪、风化岩石以及朽木样品,预处理后使用不同方法进行分离纯化,观察分离粘细菌的营养细胞、粘孢子、子实体和菌落形态等特征,并进行鉴定归属。[结果]共分离纯化得到42株粘细菌,鉴定为粘球菌属、囊球粘细菌属、粒状粘细菌属、堆囊粘细菌属、蜂窝粘细菌属、标桩菌属和原囊粘细菌属,共7个属。其中,水样中粘细菌的出菌率最高。[结论]水样中有丰富的粘细菌资源。[Objective] To lay the basis for further studying bioactive substances in myxobacteria.[Method] A total of 35 samples including fresh water,mud,rabbit dung,sheep dung,weathered rock and rotten wood were collected from districts and counties of Chengdu.After pretreatment,the myxobacteria were isolated and purified using different methods.Vegetative cells,myxospores,fruiting body and colony morphology of these myxobacteria strains were observed.[Result] Forty-two myxobacteria strains were isolated and purified.They were classified into seven genera including Myxococcus,Angiococcus,Chondromyces,Sorangium,Melittangium,Stigmatella and Archangium.The positive rate of myxobacteria was highest in the water samples.[Conclusion]Abundant myxobacteria resource is found in fresh water.

关 键 词：粘细菌 分离 纯化 菌株 

分 类 号：S188[农业科学—农业基础科学]