益生菌酪酸菌CB-7的生物学特性研究  

Study on the Biological Characteristics of Probiotics Clostridium butyricum CB-7

作　　者：陈秋红[1,2] 孙梅[1,2] 匡群 施大林[1,2] 张维娜[1,2] 刘淮 胡凌虹 何义进[1] 谢骏[1] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,江苏无锡214081 [2]江苏省苏微微生物研究有限公司,江苏无锡214063

出　　处：《安徽农业科学》2011年第10期5922-5925,共4页Journal of Anhui Agricultural Sciences

基　　金：农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金项目(BZ2009-13);江苏省科技计划项目(科技支撑计划--农业部分;BE2009372);无锡市科技支撑项目(农业攻关;CMEN0904)

摘　　要：[目的]考察酪酸菌CB-7的生物学特性。[方法]对菌株的耐酸、耐胆盐、耐高温性能及其对动物肠道致病菌大肠杆菌、志贺氏菌、沙门氏菌的抑制作用,对肠道有益菌嗜酸乳杆菌、双歧杆菌和粪链球菌生长的促进作用进行了研究。[结果]酪酸菌CB-7在pH值为3.0的培养基中37℃培养4 h后,存活率达90.0%;在含0.3%牛胆盐的培养基中37℃培养4 h后,存活率达到91.8%;经90℃水浴处理10 min、100℃水浴处理5 min后,存活率均在90.0%以上;对大肠杆菌、志贺氏菌、沙门氏菌均有显著的抑制作用,其发酵提取物可显著促进嗜酸乳杆菌、双歧杆菌和粪链球菌的生长。[结论]酪酸菌CB-7具有较强的耐酸、耐胆盐和耐高温性能,对肠道致病菌具有较强的抑制作用,且可显著促进肠道有益菌的生长,因此是一株性能优良的饲用菌株。[Objective] To investigate the biological characteristics of Clostridium butyricum CB-7.[Method] The tests of acid-tolerance,bile salt-tolerance and high temperature-tolerance of Clostridium butyricum CB-7 were performed.In addition,the effects of Clostridium butyricum CB-7 on enteropathogenic bacteria like E.coli,Shigella and Salmonella as well as the intestinal beneficial bacteria like Lactobacillus acidophilus,Bifidobacterium,and Enterococcus faecalis.[Result] After culturing for 4 h at 37 ℃（pH 3.0）,the survival rate of the Clostridium butyricum CB-7 was 90.0%;and the survival rate was 91.8% when they were cultured in medium containing 0.3% bile salt at 37 ℃ for 4 h.After water bathing at 90 ℃ for 10 min or 100 ℃ for 5 min,the survival rate of the Clostridium butyricum CB-7 was over 90.0%.The Clostridium butyricum CB-7 had great inhibition against E.coli,Shigella and Salmonella,and its extracts could greatly promote the growth of Lactobacillus acidophilus,Bifidobacterium,and Enterococcus faecalis.[Conclusion] The Clostridium butyricum CB-7 has strong resistance to acid,bile salt and high temperature,and strong inhibition against enteropathogenic bacteria,and it can also promote growth of intestinal beneficial bacteria.Thus,the Clostridium butyricum CB-7 is a feed strain with excellent performance.

关 键 词：酪酸菌 生物学特性 存活率 致病菌 有益菌 

分 类 号：S816.7[农业科学—饲料科学]