生防木霉菌T_2菌株对禾草腐霉病抑菌作用及机制研究  

Antagonism and mechanism of action of Trichoderma aureoviride against Pythium aphanidermatum causing turfgrass root rot

作　　者：古丽君[1] 徐秉良[1] 梁巧兰[1] 李荣峰[1] 

机构地区：[1]甘肃农业大学草业学院植物病理系草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业学报》2011年第2期46-51,共6页Acta Prataculturae Sinica

基　　金：草业生态系统教育部省部共建重点实验室项目(CY-GG-2006-013);甘肃省农牧厅生物技术专项(GNSW-2009-04);甘肃省教育厅项目(042-03)资助

摘　　要：深绿木霉对引起禾草腐霉病原菌瓜果腐霉有明显的抑制作用。深绿木霉菌与瓜果腐霉对峙培养结果表明,深绿木霉菌可以包围、覆盖瓜果腐霉的菌落,同时产生大量孢子;当孢子悬浮液的浓度为4.65×106个/mL时,对瓜果腐霉菌的抑制率达53.56%。通过深绿木霉菌孢子悬浮液和3种药剂对草坪禾草腐霉病病原菌抑制效果的比较,深绿木霉孢子悬浮液浓度为1×107个/mL时与25%甲霜.霜霉的抑菌效果接近,分别为77.37%和79.57%。显微观察结果表明,深绿木霉菌丝缠绕在瓜果腐霉菌菌丝上,穿透菌丝在其内生长;或与瓜果腐霉菌的菌丝平行生长,再侵入瓜果腐霉菌内寄生。深绿木霉菌对瓜果腐霉的主要作用机制为重寄生作用。In agar culture,Trichoderma aureoviride inhibited the growth of Pythium aphanidermatum,the cause of turfgrass root rots.It covered the P.aphanidermatum colony and produced large numbers of conidia.The effective concentration was at 4.65×106 cfu/mL,and the inhibitory effect reached 53.56%.Comparing the inhibition effect of T.aureoviride suspension with that of three kinds of fungicides,T.aureoviride at a concentration of 107 cfu/mL had the same effect as 25% metalaxyl with inhibition rates of 77.37% and 79.57%,respectively.Microscopic observation showed that T.aureoviride either entwined the hyphae of P.aphanidermatum or grew parallel with them,then penetrated and parasitized them.The main antagonistic mechanism of T.aureoviride against P.aphanidermatum was hyperparasitism.

关 键 词：深绿木霉 草坪禾草腐霉病病菌 抑菌效果 拮抗机制 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]