职业接触苯乙烯工人静脉血中血红蛋白-环氧苯乙烯加合物的分析测定  

DETECTION OF HEMOGLOBIN-STYRENE OXIDE ADDUCTS IN HUMAN BLOOD EXPOSED TO STYRENE

作　　者：刘淑芬[1] 方清明[1] 金祖亮[1] RappaportSM 

机构地区：[1]中国科学院生态环境研究中心,北京100085 [2]UNCATCHAPELHILLNCUSA

出　　处：《环境化学》1999年第5期445-452,共8页Environmental Chemistry

基　　金：国家自然科学基金资助项目(批准号:59873028);美国职业与健康基金资助

摘　　要：人的静脉血中血红蛋白(Hb)-环氧苯乙烯(SO)加合物的分析测定过程是:从人的静脉血中提取的血红蛋白在一定条件下酶解,蛋白质中半胱氨酸残留加合物再经Raney Nickel催化反应生成两种加合物的异构体:α-苯己醇(2-PE),β-苯乙醇(1-PE),再用五氟苯酰氯衍生化,最后用GC/MS测定这两种加合物的含量,同样方法测SD大鼠的血红蛋白-SO加合物(in vitro和in vivo).实验结果表明:在动物实验中,所测的血红蛋白-环氧苯乙烯两种加合物(I-PE,2-PE)与实验的苯乙烯或SO剂量有较好的相关性;且2-PE>1-PE,说明氧化苯乙烯与半胱氨酸的反应α位强于β位.在人群实验中,对一石棉厂职业接触苯乙烯工人(11人)静脉血中的血红蛋白-环氧苯乙烯加合物进行测定,结果是α位的(2-PE),α位+β位(2-PE+1-PE)的加合物与个体接触苯乙烯浓度有较好的相关性;而β位的加合物不明显,实验结果为探索用人的血红蛋白中的半胱氨酸-环氧苯乙烯加合物作为人接触苯乙烯的生物标志物提供了可靠的现场实验依据.An assay is described for measures of adducts of styrene oxide (SO), with molecules of hemoglobin (Hb) which have been modified at residues containing sulhydryl group. Sample of containing SO-cystein adducts are hydrolyzed, then and reacted with a reductive catalyst ( Raney Nickel) to liberate the two position isomer of SO-cysteine adducts, i. e.,1-phenylethanol (1-PE) and 2-phenylethanol (2-PE). The liberated products are derivatized with pentafluorobenzoyl chloride and measured by GC/MS. The assay was evaluated with SO adducts of Hb with had been produced in blood of SD rat (in vitro, in vivo ) and in human blood exposed styrene Level of SO-Hb adducts (1-PE, 2-PE) in rat increased with dose in hemoglobin and the a -position rather that the β-position both. The two SO-Hb adducts (1-PE, 2-PE,) were found in workers blood exposed to styrene and there is a good relativity between adduct (2-PE,1-PE+ 2PE) level and exposed dose of styrene. It was suggested that SO-Hb adduct could be a biomarker of styrene exposure in workers.

关 键 词：蛋白质加合物 环氧 苯乙烯 静脉血 职业病 

分 类 号：R135.12[医药卫生—劳动卫生]